分光光度法测定纯品、片剂和安瓿剂中地西泮的含量。
Spectrophotometric Determination of Diazepam in Pure form, Tablets and Ampoules.
作者信息
El-Hawary W F, Issa Y M, Talat A
机构信息
Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
出版信息
Int J Biomed Sci. 2007 Mar;3(1):50-5.
The interaction of diazepam with picric acid (I), 3,5-dinitrobenzoic acid (II) and 2,4-dinitrobenzoic acid (III) was found to be useful for its spectrophotometric determination. The quantitation was carried out at 475, 500, and 500 nm for the reaction with (I), (II) and (III), respectively. The effect of several variables on the coloring process was studied. The proposed methods have been applied successfully for the determination of diazepam in pure samples and in its pharmaceutical preparations with good accuracy and precision. The results were compared to those obtained by the pharmacopoeial methods. The linear ranges for obedience of Beer's law are up to 85.6, 180.2, and 128.6 µg/ml, Ringbom ranges are 10.0-79.0, 15.2-177.8, 17.0-83.0 µg/ml, and RSD 0.048, 0.028, and 0.026% for reaction of diazepam with I, II, and III, respectively.
已发现地西泮与苦味酸（I）、3,5 - 二硝基苯甲酸（II）和2,4 - 二硝基苯甲酸（III）的相互作用可用于其分光光度法测定。分别在475、500和500 nm处对与（I）、（II）和（III）的反应进行定量。研究了几个变量对显色过程的影响。所提出的方法已成功应用于纯样品及其药物制剂中地西泮的测定，具有良好的准确度和精密度。将结果与药典方法获得的结果进行了比较。符合比尔定律的线性范围分别高达85.6、180.2和128.6 μg/ml，林邦范围分别为10.0 - 79.0、15.2 - 177.8、17.0 - 83.0 μg/ml，地西泮与I、II和III反应的相对标准偏差分别为0.048%、0.028%和0.026%。
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