Umapathi P, Parimoo P
Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Rajasthan State, India.
J Pharm Biomed Anal. 1995 Jul;13(8):1003-9. doi: 10.1016/0731-7085(95)01316-d.
The determination of imipramine HCl and diazepam in tablets by derivative spectrophotometry is described. The drugs in combined preparations have been quantified using the second-order derivative spectra of their solutions in 0.1 M HCl. The method has been applied to pure drug mixtures as well as commercial preparations and was found to be precise and reproducible. Compliance of Beer's Law was observed in the concentration range of 10-70 micrograms ml-1 for imipramine HCl and 2-8 micrograms ml-1 for diazepam. Lower limits of detection at the 95% confidence level were 1.96 micrograms ml-1 for imipramine HCl and 0.21 microgram ml-1 for diazepam.
本文描述了用导数分光光度法测定片剂中盐酸丙咪嗪和地西泮的方法。采用它们在0.1M盐酸溶液中的二阶导数光谱对复方制剂中的药物进行了定量。该方法已应用于纯药物混合物以及市售制剂，结果表明该方法准确且可重复。盐酸丙咪嗪在10 - 70微克/毫升浓度范围内、地西泮在2 - 8微克/毫升浓度范围内符合比尔定律。在95%置信水平下，盐酸丙咪嗪的检测下限为1.96微克/毫升，地西泮的检测下限为0.21微克/毫升。
