Balcerczak E, Balcerczak M, Mirowski M
Department of Pharmaceutical Biochemistry, Laboratory of Molecular Biology and Pharmacogenomics, Lodz, Poland;
Int J Biomed Sci. 2007 Dec;3(4):287-91.
P65 gene expression level was determined in colon cancer cases by means of real-time PCR. 51 cases of colorectal carcinomas showing positive RT-PCR signals for P65 gene expression selected from 109 frozen samples were further investigated by quantitative real-time PCR. P65 levels were higher in cancer with metastases to lymph nodes and distant metastases. Higher levels were observed in more advanced cases classified as III and IV according to pTNM classification. In two groups of patients with vessel invasion and absence of lymphocytes in tumour tissue, the presence of P65 expression correlated with shorter survival time. Quantitative results confirmed that P65 gene expression in colon cancer is engaged in the process of metastasis formation and could be correlated with worse prognosis for the patients.
通过实时定量聚合酶链反应（PCR）测定结肠癌病例中P65基因的表达水平。从109份冷冻样本中选取51例P65基因表达呈逆转录聚合酶链反应（RT-PCR）阳性信号的结直肠癌病例，进一步通过定量实时PCR进行研究。P65水平在伴有淋巴结转移和远处转移的癌症中更高。根据pTNM分类，在更晚期的III期和IV期病例中观察到更高的水平。在两组肿瘤组织中有血管侵犯且无淋巴细胞的患者中，P65表达的存在与较短的生存时间相关。定量结果证实，结肠癌中P65基因表达参与转移形成过程，并且可能与患者预后较差相关。
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