p65基因在结直肠癌患者中的表达：与某些组织学类型、分级及临床分期的比较

Expression of the p65 gene in patients with colorectal cancer: comparison with some histological typing, grading and clinical staging.

作者信息

Balcerczak M, Balcerczak E, Pasz-Walczak G, Kordek R, Mirowski M

机构信息

Molecular Biology Laboratory, Department of Pharmaceutical Biochemistry, Medical University, Muszynskiego 1 Street, 90-151 Lodz, Poland.

出版信息

Eur J Surg Oncol. 2004 Apr;30(3):266-70. doi: 10.1016/j.ejso.2003.11.006.

DOI:10.1016/j.ejso.2003.11.006

PMID:15028307

Abstract

AIMS

P65 protein/gene is a potential, non-specific tumour marker expressed by different types of neoplasms. In this study p65 gene expression was estimated in a group of colorectal cancers and compared with some histological features, grading and clinical staging of the neoplasms to assess its role as a prognostic marker for colorectal cancer.

METHODS

109 pairs of frozen samples of colorectal carcinomas and adjacent normal colorectal mucosa and four samples of tissue from patients without neoplastic diseases were analysed by means of RT-PCR.

RESULTS

Expression of p65 gene was found in 51 out of 109 cases of colorectal cancers. For 19 of them expression of examined gene was observed both in cancer and corresponding healthy mucosa. p65 Gene expression was associated with more advanced tumours (T3, T4; p=0.0003) with metastases to lymph nodes (N1, N2; p=0.0003) and distant metastases (p=0.0005). We did not find association between the age, gender, tumour localization, histological type and p65 gene expression.

CONCLUSIONS

p65 Gene expression in primary tumour tissue is associated with poor prognosis for the patients with colorectal cancer in this series.

摘要

目的

P65蛋白/基因是一种由不同类型肿瘤表达的潜在非特异性肿瘤标志物。在本研究中，对一组结直肠癌患者的p65基因表达进行了评估，并与肿瘤的一些组织学特征、分级和临床分期进行比较，以评估其作为结直肠癌预后标志物的作用。

方法

采用逆转录聚合酶链反应（RT-PCR）对109对结直肠癌冷冻样本及相邻正常结直肠黏膜样本，以及4例无肿瘤疾病患者的组织样本进行分析。

结果

109例结直肠癌患者中，51例检测到p65基因表达。其中19例在癌组织及相应的健康黏膜中均检测到该基因表达。p65基因表达与更晚期肿瘤（T3、T4；p = 0.0003）、有淋巴结转移（N1、N2；p = 0.0003）及远处转移（p = 0.0005）相关。未发现年龄、性别、肿瘤部位、组织学类型与p65基因表达之间存在关联。