Kligman L H, Mathews-Roth M M
University of Pennsylvania, School of Medicine, Department of Dermatology, Philadelphia 19104-6142.
Photochem Photobiol. 1990 Jun;51(6):733-5.
Albino hairless mice were fed diets containing 10 g/kg feed of beta-carotene and 200 mg/kg feed of 13-cis retinoic acid to assess the ability of these molecules to prevent UVB-induced dermal damage. Diets were administered for 12 weeks prior to UVB exposure and were continued throughout the 20 week irradiation period. The UVB source was a bank of FS-20 sunlamps (280-400 nm: peak 313 nm). Exposures were thrice weekly at 0.1 J/cm2 per exposure for the first 10 weeks and 0.2 J/cm2 per exposure for the second 10 weeks. Histologic evaluation of skin biopsies revealed no difference, between animals fed active or placebo diets, in UVB-induced elastosis, collagen changes or amounts of glycosaminoglycans and proteoglycans of the ground substance.
给白化无毛小鼠喂食含10克/千克饲料的β-胡萝卜素和200毫克/千克饲料的13-顺式视黄酸，以评估这些分子预防紫外线B（UVB）诱导的皮肤损伤的能力。在UVB照射前12周给予饲料，并在整个20周的照射期内持续喂食。UVB光源是一组FS-20太阳灯（280 - 400纳米：峰值313纳米）。在前10周，每周照射三次，每次照射剂量为0.1焦耳/平方厘米，后10周每次照射剂量为0.2焦耳/平方厘米。对皮肤活检组织的组织学评估显示，喂食活性饲料或安慰剂饲料的动物在UVB诱导的弹性组织变性、胶原蛋白变化或基质中糖胺聚糖和蛋白聚糖的含量方面没有差异。
