Institute for Health and Life Sciences, Tokyo Medical & Dental University Open Laboratory, Medical Research Institute Surugadai Bldg, 2-3-10, Surugadai, Kanda, Chiyoda-ku, Tokyo 101-0062, Japan.
Immun Ageing. 2013 May 15;10(1):19. doi: 10.1186/1742-4933-10-19.
Gender-related differences in humans are commonly observed in behaviour, physical activity, disease, and lifespan. However, the notion that age-related changes in the immune system differ between men and women remains controversial. To elucidate the relationship between immunological changes and lifespan, peripheral blood mononuclear cells from healthy Japanese subjects (age range: 20-90 years; N = 356) were analysed by using three-colour flow cytometry. The proliferative activities and cytokine-producing capacities of T cells in response to anti-CD3 monoclonal antibody stimulation were also assessed.
An age-related decline in the number of T cells, certain subpopulations of T cells (including CD8+ T cells, CD4+CDRA+ T cells, and CD8+CD28+ T cells), and B cells, and in the proliferative capacity of T cells was noted. The rate of decline in these immunological parameters, except for the number of CD8+ T cells, was greater in men than in women (p < 0.05). We observed an age-related increase or increasing trend in the number of CD4+ T cells, CD4+CDRO+ T cells, and natural killer (CD56+CD16+) cells, as well as in the CD4+ T cell/CD8+ T cell ratio. The rate of increase of these immunological parameters was greater in women than in men (p < 0.05). T cell proliferation index (TCPI) was calculated from the T cell proliferative activity and the number of T cells; it showed an age-related decline that was greater in men than in women (p < 0.05). T cell immune score, which was calculated using 5 T cell parameters, also showed an age-related decline that was greater in men than in women (p < 0.05). Moreover, a trend of age-related decreases was observed in IFNγ, IL-2, IL-6, and IL-10 production, when lymphocytes were cultured with anti-CD3 monoclonal antibody stimulation. The rate of decline in IL-6 and IL-10 production was greater in men than in women (p < 0.05).
Age-related changes in various immunological parameters differ between men and women. Our findings indicate that the slower rate of decline in these immunological parameters in women than that in men is consistent with the fact that women live longer than do men.
人类的行为、身体活动、疾病和寿命方面普遍存在与性别相关的差异。然而，关于免疫系统的年龄相关性变化在男性和女性之间存在差异的观点仍存在争议。为了阐明免疫变化与寿命之间的关系，使用三色流式细胞术分析了来自健康的日本受试者（年龄范围：20-90 岁；N=356）的外周血单个核细胞。还评估了 T 细胞对抗 CD3 单克隆抗体刺激的增殖活性和细胞因子产生能力。
观察到 T 细胞数量、某些 T 细胞亚群（包括 CD8+T 细胞、CD4+CDRA+T 细胞和 CD8+CD28+T 细胞）和 B 细胞数量以及 T 细胞增殖能力随年龄的下降。除 CD8+T 细胞数量外，这些免疫参数的下降速度在男性中大于女性（p<0.05）。我们观察到 CD4+T 细胞、CD4+CDRO+T 细胞和自然杀伤（CD56+CD16+）细胞数量以及 CD4+T 细胞/CD8+T 细胞比值随年龄的增加或增加趋势。这些免疫参数的增加速度在女性中大于男性（p<0.05）。T 细胞增殖指数（TCPI）是从 T 细胞增殖活性和 T 细胞数量计算得出的；它显示出与年龄相关的下降，在男性中比女性更明显（p<0.05）。使用 5 个 T 细胞参数计算的 T 细胞免疫评分也显示出与年龄相关的下降，在男性中比女性更明显（p<0.05）。此外，当用抗 CD3 单克隆抗体刺激培养淋巴细胞时，观察到 IFNγ、IL-2、IL-6 和 IL-10 产生与年龄相关的下降趋势。男性中 IL-6 和 IL-10 产生的下降速度大于女性（p<0.05）。
男性和女性之间各种免疫参数的年龄相关性变化不同。我们的发现表明，女性的这些免疫参数下降速度比男性慢，这与女性寿命比男性长的事实一致。
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