Legarraga Paulette, Moraga Marcela, Lam Marusella, Geoffroy Enrique, Zumarán Cecilia, García Patricia
Departamento de Laboratorios Clínicos, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Rev Chilena Infectol. 2013 Apr;30(2):140-6. doi: 10.4067/S0716-10182013000200004.
MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization -Time of Flight Mass Spectrometry) technology, recently introduced in the microbiology laboratory has proven to be a precise and rapid method for bacterial identification.
To evaluate the performance, costs associated and turnaround time of MALDI-TOF in a routine laboratory.
Five hundred and sixty one clinical isolates (281 aerobes and 280 anaerobes) previously identified by conventional methods were evaluated. Discordances were resolved by means of 16S rRNA sequencing.
MALDI-TOF identified 95, 7% of the aerobes isolates and 86, 4% of the anaerobes. The groups with better performance were the enterobacteriacea and Bacteroides spp with 95% and 100% identification at the species level. The error rate of MALDI-TOF and conventional methods compared to sequencing was 0, 39% and 9, 4% respectively. The costs associated were 8 times lower with a turnaround time of 6 hours.
MALDI-TOF proved to be simple, precise and less expensive technology compared to the traditional methods.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术最近被引入微生物实验室，已被证明是一种精确、快速的细菌鉴定方法。
评估MALDI-TOF在常规实验室中的性能、相关成本和周转时间。
对先前通过传统方法鉴定的561株临床分离株（281株需氧菌和280株厌氧菌）进行评估。通过16S rRNA测序解决不一致问题。
MALDI-TOF鉴定出95.7%的需氧菌分离株和86.4%的厌氧菌分离株。性能较好的组是肠杆菌科和拟杆菌属，在种水平上的鉴定率分别为95%和100%。与测序相比，MALDI-TOF和传统方法的错误率分别为0.39%和9.4%。相关成本降低了8倍，周转时间为6小时。
与传统方法相比，MALDI-TOF被证明是一种简单、精确且成本较低的技术。
