Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan.
Chem Commun (Camb). 2013 Jun 21;49(49):5592-4. doi: 10.1039/c3cc41680a.
A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.
我们开发了一种新的蛋白激酶检测方法，基于 QD 和荧光标记底物肽之间的 FRET。带负电荷的 QD 可以识别磷酸化过程中肽净电荷的变化。尽管其机制简单，但该检测方法足够灵敏和稳健，可用于评估蛋白激酶抑制剂。
