Li Bei-Xu, Li Jia, Luo Cheng-Liang, Zhang Ming-Chang, Li Hui, Li Li-Liang, Xu Hong-Fei, Shen Yi-Wen, Xue Ai-Min, Zhao Zi-Qin
Department of Forensic Medicine, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China.
Indian J Exp Biol. 2013 Mar;51(3):208-17.
Jumonji Domain Containing 2A (JMJD2A) may be a cancer-associated gene involved in human breast cancer. With a view to investigating expression of JMJD2A in human breast cancer and benign lesion tissues as well as relationship between JMJD2A and tumor related proteins, histological and immunohistochemical analysis, Western blot and quantitative real-time PCR in infiltrating duct carcinoma and fibroadenoma for JMJD2A and immunohistochemical analysis and quantitative real-time PCR in infiltrating duct carcinoma for tumor related proteins (ARHI, p53, ER, PR and CerbB-2) were performed. Histological examination validated the clinical diagnosis. The JMJD2A positive rate of infiltrating duct carcinoma was significantly higher than fibroadenoma by immunohistochemical analysis. The mean optical density of JMJD2A in infiltrating duct carcinoma was higher than fibroadenoma by western blot. JMJD2A mRNA level in infiltrating duct carcinoma was higher than fibroadenoma by quantitative real-time PCR. Spearman correlation analysis revealed that the expression of JMJD2A was associated with ARHI, p53 and ER from immunohistochemical results respectively. Pearson correlation analysis revealed that the expression of JMJD2A was associated with ARHI, p53 and ER from quantitative real-time PCR results respectively. Expression of JMJD2A in infiltrating duct carcinoma was higher, and associated with ARHI, p53 and ER. The results may take JMJD2A as a potential diagnostic and therapeutic target in human breast cancer.
含Jumonji结构域蛋白2A（JMJD2A）可能是一种与人类乳腺癌相关的癌症相关基因。为了研究JMJD2A在人类乳腺癌和良性病变组织中的表达以及JMJD2A与肿瘤相关蛋白之间的关系，我们对浸润性导管癌和纤维腺瘤进行了组织学和免疫组织化学分析、蛋白质免疫印迹法以及针对JMJD2A的定量实时聚合酶链反应，并对浸润性导管癌进行了针对肿瘤相关蛋白（ARHI、p53、雌激素受体、孕激素受体和人表皮生长因子受体2）的免疫组织化学分析和定量实时聚合酶链反应。组织学检查验证了临床诊断。免疫组织化学分析显示，浸润性导管癌的JMJD2A阳性率显著高于纤维腺瘤。蛋白质免疫印迹法显示，浸润性导管癌中JMJD2A的平均光密度高于纤维腺瘤。定量实时聚合酶链反应显示，浸润性导管癌中JMJD2A mRNA水平高于纤维腺瘤。Spearman相关性分析显示，免疫组织化学结果表明JMJD2A的表达分别与ARHI、p53和雌激素受体相关。Pearson相关性分析显示，定量实时聚合酶链反应结果表明JMJD2A的表达分别与ARHI、p53和雌激素受体相关。JMJD2A在浸润性导管癌中的表达较高，且与ARHI、p53和雌激素受体相关。这些结果可能使JMJD2A成为人类乳腺癌潜在的诊断和治疗靶点。
