[苏云金芽孢杆菌中SigmaK和GerE对芽孢外壁基底层结构基因exsB的转录调控]
[Transcriptional regulation of exosporium basal layer structural gene exsB in Bacillus thuringiensis by SigmaK and GerE].
作者信息
Qu Ning, Peng Qi, Qiu Lili, Liu Chunxia, Chen Zhen, Zhang Jie, Song Fuping, Li Jie
机构信息
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
出版信息
Wei Sheng Wu Xue Bao. 2013 Mar 4;53(3):241-8.
OBJECTIVE
To identify the transcriptional regulation of exosporium basal layer structural gene exsB in Bacillus thuringiensis.
METHODS
We analyzed exsB and its promoter sequence in Bacillus cereus group by sequence alignment. We performed beta-galactosidase assay of exsB-lacZ gene fusion to analyze transcriptional activation of exsB promoter; we used Electrophoretic mobility shift assays to detect binding of GerE and exsB promoter.
RESULTS
exsB was the high similarity in Bacillus cereus group strains. Beta-galactosidase assay showed that exsB promoter had the strong transcriptional activation on the late sporulation phase. Deletion of gerE or sigK gene decreased the activation of exsB promoter. Electrophoretic mobility shift assays showed that GerE could bind with exsB promoter.
CONCLUSION
The exsB gene is regulated by SigmaK and GerE on the late sporulation phase.
目的
鉴定苏云金芽孢杆菌中芽孢外壁基底层结构基因exsB的转录调控。
方法
通过序列比对分析蜡样芽孢杆菌群中的exsB及其启动子序列。对exsB-lacZ基因融合体进行β-半乳糖苷酶分析，以分析exsB启动子的转录激活；使用电泳迁移率变动分析来检测GerE与exsB启动子的结合。
结果
exsB在蜡样芽孢杆菌群菌株中具有高度相似性。β-半乳糖苷酶分析表明，exsB启动子在芽孢形成后期具有强烈的转录激活作用。gerE或sigK基因的缺失降低了exsB启动子的激活。电泳迁移率变动分析表明，GerE可与exsB启动子结合。
结论
exsB基因在芽孢形成后期受SigmaK和GerE调控。
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