Departamento de Morfologia e Ciências Fisiolígicas, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Pará, Av. Hiléia Agrópolis do INCRA s/n, 68503-120 Marabá, Brazil.
Genes Brain Behav. 2013 Jul;12(5):576-82. doi: 10.1111/gbb.12047. Epub 2013 Jun 17.
The zebrafish leopard phenotype (leo) displays abnormal pigmentation and shows increased anxiety-like behavior. The neurochemical changes associated with this anxious phenotype are not known. Here, we demonstrate that leo show increased anxiety-like behavior in the light/dark box and in the novel tank test. This anxious phenotype is rescued by acute treatment with a dose of a serotonin reuptake inhibitor, fluoxetine, that is inactive in wild-type animals. Moreover, leo show decreased tissue levels of serotonin, increased serotonin turnover and slightly increased monoamine oxidase activity. These results suggest that the anxious phenotype observed in leo zebrafish is caused by a decrease in serotonin uptake. This work could open an important avenue in defining the neurochemical underpinning of natural variation in anxiety disorders.
斑马鱼豹纹表型（leo）显示出异常的色素沉着，并表现出增加的类似焦虑的行为。与这种焦虑表型相关的神经化学变化尚不清楚。在这里，我们证明 leo 在明暗箱和新鱼缸测试中表现出增加的类似焦虑的行为。这种焦虑表型可以通过急性给予剂量的 5-羟色胺再摄取抑制剂氟西汀来挽救，而在野生型动物中氟西汀是无效的。此外，leo 显示出组织中 5-羟色胺水平降低、5-羟色胺周转率增加和单胺氧化酶活性略有增加。这些结果表明，在 leo 斑马鱼中观察到的焦虑表型是由 5-羟色胺摄取减少引起的。这项工作可能为确定焦虑症自然变异的神经化学基础开辟了一条重要途径。
