Department of Food Science, Massachusetts Agricultural Experiment Station, University of Massachusetts, Amherst, MA 01003.
Department of Food Science, Massachusetts Agricultural Experiment Station, University of Massachusetts, Amherst, MA 01003.
J Microbiol Methods. 2013 Aug;94(2):69-72. doi: 10.1016/j.mimet.2013.05.001. Epub 2013 May 13.
Foods contaminated with pathogens are common sources of illness. Currently, the most common and sensitive rapid detection method involves the PCR. However, food matrices are complex and contain inhibitors that limit the sensitivity of the PCR. The use of coated activated carbon can effectively facilitate the removal of PCR inhibitors without binding targeted bacterial cells from food samples. With the use of activated carbon coated with milk proteins, a cell recovery at pH 7.0 of 95.7%±2.0% was obtained, compared to control uncoated activated carbon, which yielded a cell recovery of only 1.1%±0.8%. In addition, the milk protein coated activated carbon was able to absorb similar amounts of soluble compounds as uncoated activated carbon, with the exception of bovine hemoglobin. This suggests that the use of milk proteins to coat activated carbon may therefore serve as a suitable replacement for bentonite in the coating of activated carbon, which has previously been used for the removal of PCR inhibitors from food.
受病原体污染的食物是疾病的常见来源。目前，最常见和最敏感的快速检测方法涉及聚合酶链式反应（PCR）。然而，食物基质较为复杂，其中含有抑制物，限制了 PCR 的灵敏度。使用涂覆有活性炭可以有效地促进 PCR 抑制剂的去除，而不会从食物样本中结合靶向细菌细胞。使用涂覆有牛奶蛋白的活性炭，在 pH 值为 7.0 时可获得 95.7%±2.0%的细胞回收率，而对照的未涂覆活性炭的细胞回收率仅为 1.1%±0.8%。此外，涂覆有牛奶蛋白的活性炭能够吸收与未涂覆活性炭相似量的可溶性化合物，除了牛血红蛋白。这表明，使用牛奶蛋白涂覆活性炭可能是替代先前用于从食物中去除 PCR 抑制剂的活性炭涂层中的膨润土的合适选择。
