Department of Mechanical Engineering, Southern Taiwan University of Science and Technology, Tainan 71005, Taiwan.
Biosens Bioelectron. 2013 Oct 15;48:158-64. doi: 10.1016/j.bios.2013.03.064. Epub 2013 Apr 10.
A novel modification of low-cost Al₂O₃ nanoparticles (Al₂O₃ NPs) for antibody-protein immunosensing is proposed. The modified NPs are utilized to enhance the intensity of fluorescence in a dielectrophoretic (DEP) chip with a microelectrode array. The surface of the Al₂O₃ NPs is modified by ionic polyaniline (PANDB) rather than the conventional silane (3-aminopropyltrimethoxysilane) to conjugate the antibody on the outer shell. After the PANDB-Al₂O₃ NPs is functionalized to form probes, a DEP chip with a vertical non-uniform electric field that is produced by top and bottom electrodes condenses and immobilizes the nanoprobes on the surface of the electrodes by positive DEP force for immunosensing of the fluorescent protein. Additionally, each microelectrode array can be individually controlled with/without DEP manipulation using a computer program. Experimental results indicate that PANDB-based nanoprobes provide more rapid and sensitive immunosensing than those having undergone conventional silane modification. During immunosensing, fluorescence intensity can be doubled by the application of extra DEP force. The individual control of NPs on the microelectrode array has great potential for applications in multi-antibody arrays in a single chip for immunosensing.
提出了一种新颖的低成本 Al₂O₃ 纳米粒子 (Al₂O₃ NPs) 修饰方法，用于抗体-蛋白质免疫传感。修饰后的 NPs 用于增强带有微电极阵列的介电泳 (DEP) 芯片中的荧光强度。Al₂O₃ NPs 的表面通过离子聚苯胺 (PANDB) 进行修饰，而不是传统的硅烷 (3-氨丙基三甲氧基硅烷)，以在外壳上结合抗体。在 PANDB-Al₂O₃ NPs 功能化形成探针后，由顶部和底部电极产生的垂直非均匀电场的 DEP 芯片通过正 DEP 力将纳米探针凝聚并固定在电极表面上，用于荧光蛋白的免疫传感。此外，每个微电极阵列都可以使用计算机程序进行有/无 DEP 操作的单独控制。实验结果表明，基于 PANDB 的纳米探针比经过传统硅烷修饰的探针提供更快和更敏感的免疫传感。在免疫传感过程中，通过施加额外的 DEP 力可以使荧光强度增加一倍。在微电极阵列上对 NPs 进行单独控制，在单个芯片中用于多抗体阵列的免疫传感方面具有很大的应用潜力。
