发育中鸡胚的基因转移：卵内电穿孔

Gene transfer in developing chick embryos: in ovo electroporation.

作者信息

Farley Emma K

机构信息

Division of Genetics, Genomics and Development Center for Integrative Genomics, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

出版信息

Methods Mol Biol. 2013;1018:141-50. doi: 10.1007/978-1-62703-444-9_14.

DOI:10.1007/978-1-62703-444-9_14

PMID:23681625

Abstract

In ovo electroporation is a popular technique to study gene function during development. This technique enables precise temporal and spatial genetic manipulation with the added advantages of being quick and inexpensive. In this chapter the transient transfection of a construct into the neural tube of a chicken embryo via in ovo electroporation is described. Modifications of this basic technique and methods to -analyze the resulting electroporated embryos such as qPCR and microarray are also discussed.

摘要

卵内电穿孔是一种在发育过程中研究基因功能的常用技术。该技术能够实现精确的时空基因操作，还具有快速且成本低的额外优势。在本章中，将描述通过卵内电穿孔将构建体瞬时转染到鸡胚神经管中的方法。此外，还将讨论对这一基本技术的改进以及分析由此产生的电穿孔胚胎的方法，如定量聚合酶链反应（qPCR）和微阵列分析。