Namavari M, Oryan A, Namazi F, Kargar M, Mansourian M, Rahimian A, Tahamtan Y
Razi Vaccine and Serum Research Institute, Shiraz, Iran.
Iran J Parasitol. 2013 Jan;8(1):99-106.
Caprine besnoitiosis is an economically important disease of goats. Neospora caninum, another coccidian parasite of worldwide distribution, infects several animal species and is a major cause of abortion in cattle. Combined infections of N. caninum and Besnoitia caprae can occur in geographical areas endemic for both species of parasite in goats. This experiment was conducted to investigate the possible cross-immunity between these two infections in experimentally infected BALB/c mice.
Forty BALB/c mice were divided into four equal groups. The mice of Groups 1 and 4 were inoculated with 1×10(6) live virulent tachyzoites of N. caninum (NC-1), while animals of Groups 2 and 3 were inoculated with sterile tissue culture medium. Each mouse in Groups 1 and 2 was challenged 28 days later with 1×10(6) live virulent bradyzoites of B. Caprae (BC-1).
Following the challenge, the mice in Groups 1 and 2 showed 100% morbidity and 100% mortality within 9 days post infection, while all the animals of Groups 3 and 4 remained alive. The dead animals were necropsied. The survivors (mice in Group 3 and 4) were euthanized 9 days after inoculation and the gross and histopathological lesions in different organs were investigated.
Immunization and challenge experiments with lethal dose of B. caprae in the highly susceptible BALB/c mice showed no cross-protection between N. caninum and B. caprae.
山羊巴贝斯虫病是一种对山羊具有重要经济影响的疾病。犬新孢子虫是另一种分布于全球的球虫寄生虫，可感染多种动物，是牛流产的主要原因。在山羊中这两种寄生虫的地方性流行地区，犬新孢子虫和山羊巴贝斯虫可能会发生混合感染。本实验旨在研究在经实验感染的BALB/c小鼠中这两种感染之间可能存在的交叉免疫。
将40只BALB/c小鼠平均分为四组。第1组和第4组小鼠接种1×10(6)个活的毒力强的犬新孢子虫速殖子（NC-1），而第2组和第3组动物接种无菌组织培养基。第1组和第2组的每只小鼠在28天后用1×10(6)个活的毒力强的山羊巴贝斯虫缓殖子（BC-1）进行攻击。
攻击后，第1组和第2组的小鼠在感染后9天内发病率和死亡率均为100%，而第3组和第4组的所有动物均存活。对死亡动物进行尸检。在接种后9天对存活动物（第3组和第4组小鼠）实施安乐死，并对不同器官的大体和组织病理学病变进行研究。
在高度易感的BALB/c小鼠中用致死剂量的山羊巴贝斯虫进行免疫和攻击实验表明，犬新孢子虫和山羊巴贝斯虫之间没有交叉保护作用。
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