伊朗马什哈德采用不同诊断技术对与犬新孢子虫相关的牛流产进行评估。
Evaluation of bovine abortion associated with Neospora caninum by different diagnostic techniques in Mashhad, Iran.
作者信息
Sadrebazzaz A, Habibi G, Haddadzadeh H, Ashrafi J
机构信息
Razi Serum and Vaccine Research Institute, P.O. Box 91735-174, Mashhad, Iran.
出版信息
Parasitol Res. 2007 May;100(6):1257-60. doi: 10.1007/s00436-006-0417-3. Epub 2007 Jan 6.
Twelve aborted foetuses (gestational ranged from 4-9 months) and dams from dairies cattle farms in (Mashhad) Iran were analysed to investigate the participation of Neospora caninum in abortion. Diagnosis of the infection was determined by histopathology, serology (indirect fluorescent antibody test [IFAT]) and semi-nested polymerase chain reaction (PCR). A total 33% of bovine foetuses were considered to be infected by PCR technique. Microscopic lesions consistent with N. caninum infection in foetal brains were observed in 25% of the samples, whereas 33% were positive using IFAT (with a cut-off titre of 1:20). This study confirms the importance of N. caninum as an important cause of abortion in Iran.
对来自伊朗马什哈德奶牛场的12例流产胎儿（孕周为4 - 9个月）及其母畜进行分析，以调查犬新孢子虫在流产中的作用。通过组织病理学、血清学（间接荧光抗体试验[IFAT]）和半巢式聚合酶链反应（PCR）来确定感染的诊断。采用PCR技术，共33%的牛胎儿被认为受到感染。在25%的样本中观察到胎儿大脑中与犬新孢子虫感染相符的微观病变，而采用IFAT（临界滴度为1:20）时，33%呈阳性。本研究证实了犬新孢子虫作为伊朗流产重要病因的重要性。
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