Department of BIN Fusion Technology, Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Beackje-daero, Deokjin , Jeonju 561-756, Korea.
J Biomater Sci Polym Ed. 2013;24(9):1084-98. doi: 10.1080/09205063.2012.735100. Epub 2012 Nov 22.
Alginate, a polysaccharide extracted from brown seaweed, remains the most widely used biomaterial for immobilizing cells to be transplanted, because of the good viability of the encapsulated cells and the relatively ease of processing for cell encapsulation. However, the main drawback is the immune reaction in vivo. To overcome this problem, we have demonstrated a modified Korbutt method for alginate purification. After alginate microcapsules were manufactured, NIH/3T3 fibroblast cells were seeded in purified and non-purified alginate microcapsules, and the cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. Reverse transcriptase-polymerase chain reaction was performed to assess the mRNA expression of RAW 264.7 macrophage cells for inflammation cytokines such as TNF-α. Purified and non-purified alginate microcapsules were implanted into Wister rats, and subsequently extracted after 1-2 weeks. Tissues surrounding the implants were harvested and underwent histological evaluation through H&E staining and immunohistochemical evaluation through ED-1 staining. In this result, contaminated materials in the purified alginate were eliminated by purification process. Thereby, density of inflammatory cell decreased about 30% more than non-purified alginate and thickness of fibrotic wall decreased about three times. In concluding, the purified alginate is anticipated to be highly potent for numerous biomaterial applications.
藻酸盐是从褐藻中提取的一种多糖，仍然是最广泛用于固定移植细胞的生物材料，因为包封细胞的良好活力和相对容易进行细胞包封处理。然而，主要的缺点是体内的免疫反应。为了克服这个问题，我们已经展示了一种改良的藻酸盐纯化方法。在制造藻酸盐微胶囊后，将 NIH/3T3 成纤维细胞接种在纯化和未纯化的藻酸盐微胶囊中，并通过 3-(4,5-二甲基噻唑-2-基)-2,5 二苯基四氮唑溴盐测定法分析细胞增殖。通过逆转录-聚合酶链反应评估 RAW 264.7 巨噬细胞细胞炎症细胞因子如 TNF-α 的 mRNA 表达。将纯化和未纯化的藻酸盐微胶囊植入 Wister 大鼠体内，然后在 1-2 周后提取。收获植入物周围的组织，并通过 H&E 染色进行组织学评估，通过 ED-1 染色进行免疫组织化学评估。在这个结果中，通过纯化过程消除了纯化藻酸盐中的污染材料。因此，炎症细胞的密度比未纯化的藻酸盐降低了约 30%，纤维性壁的厚度降低了约三倍。总之，纯化的藻酸盐有望在许多生物材料应用中具有很高的潜力。
