Conservative Dentistry Department, Faculty of Dentistry, Federal University of Rio Grande do Sul, Brazil.
Arch Oral Biol. 2013 Sep;58(9):1174-9. doi: 10.1016/j.archoralbio.2013.04.001. Epub 2013 May 15.
To assess histological features and the expression of STRO-1 and BMP-4 in dental pulp and periapical tissues in vital or necrotic rat immature teeth.
The lower left first molars of male Wistar rats ageing four weeks (n=24) had their pulps exposed to the oral environment for 3, 6, 9 and 12 weeks (animals ageing 7, 10, 13 and 16 weeks-old, respectively; n=24). The right lower first molars served as control untouched teeth. After sample harvesting the jaws were dissected and processed for histology and immunodetection of STRO-1 and BMP-4.
Necrotic teeth had root development arrested, while control animals showed development of dental tissues. Immunohistochemistry showed that detection of BMP-4 was restricted to vital pulps. For both groups, STRO-1 expression was evident around blood vessels walls. Neither BMP-4 nor STRO-1 was observed in the apical papilla region.
STRO-1-positive precursor cells were not detected in the apical papilla. BMP-4 expression has not been detected during infection.
评估活髓或坏死状态下大鼠未成熟牙牙髓和根尖周组织的组织学特征，以及 STRO-1 和 BMP-4 的表达情况。
4 周龄雄性 Wistar 大鼠的左下第一磨牙牙髓暴露于口腔环境中 3、6、9 和 12 周（分别为 7、10、13 和 16 周龄的动物；n=24）。右侧下颌第一磨牙作为未受干扰的对照牙齿。采集样本后，将颌骨解剖并进行组织学和 STRO-1、BMP-4 免疫检测。
坏死牙的牙根发育停止，而对照动物的牙组织发育正常。免疫组织化学显示 BMP-4 仅在活髓中检测到。对于两组，STRO-1 的表达均明显存在于血管壁周围。在根尖乳头区域均未观察到 BMP-4 或 STRO-1。
在根尖乳头区域未检测到 STRO-1 阳性前体细胞。在感染过程中未检测到 BMP-4 的表达。
