Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Biochem Biophys Res Commun. 2013 Jun 14;435(4):586-91. doi: 10.1016/j.bbrc.2013.05.017. Epub 2013 May 17.
Fluorescent-protein transgenic mice are useful for obtaining marked somatic cells to study kinetics of development or differentiation. Fluorescence-marked hematopoietic stem cells in particular are commonly used for studying hematopoiesis. However, as far as we know, no transgenic mouse line is described in which a fluorescent protein is stably and constitutively expressed in all hematopoietic cells, including erythrocytes and platelets. Using the random segregation of provirus (RSP) method, we generated from retrovirally transduced mouse embryonic stem cells a transgenic mouse line expressing a red/orange fluorescent protein, Kusabira Orange (KuO). KuO transgenic mouse line cells carry only one proviral integration site and stably express KuO in all hematopoietic-lineage elements, including erythrocytes and platelets. Moreover, bone-marrow transplantation in KuO transgenic mice demonstrated normal hematopoieisis. KuO transgenic mice likely will prove useful for study of hematopoiesis that includes erythropoiesis and megakaryopoiesis.
荧光蛋白转基因小鼠可用于获得标记的体细胞，以研究发育或分化的动力学。特别是荧光标记的造血干细胞通常用于研究造血。然而，据我们所知，还没有描述过一种转基因小鼠品系，其中荧光蛋白在所有造血细胞（包括红细胞和血小板）中稳定且组成性表达。我们使用逆转录病毒转导的小鼠胚胎干细胞中的随机整合方法（RSP），产生了一种表达红色/橙色荧光蛋白 Kusabira Orange（KuO）的转基因小鼠品系。KuO 转基因小鼠系细胞仅携带一个前病毒整合位点，并在所有造血谱系成分中稳定表达 KuO，包括红细胞和血小板。此外，KuO 转基因小鼠的骨髓移植显示正常的造血。KuO 转基因小鼠可能将有助于包括红细胞生成和巨核细胞生成在内的造血研究。
