Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
Bioresour Technol. 2013 Jul;140:120-5. doi: 10.1016/j.biortech.2013.04.079. Epub 2013 Apr 29.
The effects of ethanol concentration gradients along with varied cultivation times on lipid and fatty acid accumulation and composition of Scenedesmus sp. were studied. The maximum increment of algal density, lipid productivity, lipid content and fatty acid content were 6.61, 11.75, 1.34 and 3.14 times higher than the control group under 12h photoperiod. Algal light deprivation inhibited ethanol positive effects on algal growth and lipid biomass. The cumulative quantity of C16:0 and C18:0 decreased correspondingly with the increase of ethanol concentrations and cultivation times. Besides, unsaturated fatty acids appeared early in algal cells and increased 57.02% in maximum. However, only 2.27% (14)C was transferred from ethanol to fatty acids. The results indicated that adding proper amount of ethanol in algal culture medium was beneficial to biodiesel feedstock production and biodiesel properties.
研究了乙醇浓度梯度以及不同培养时间对小球藻脂质和脂肪酸积累及组成的影响。在 12 小时光周期下,藻密度、产脂率、脂质含量和脂肪酸含量的最大增量分别比对照组高出 6.61、11.75、1.34 和 3.14 倍。藻类的光照剥夺抑制了乙醇对藻类生长和脂质生物量的积极影响。随着乙醇浓度和培养时间的增加,C16:0 和 C18:0 的累积量相应减少。此外,不饱和脂肪酸在藻细胞中较早出现,并在最大值时增加了 57.02%。然而,只有 2.27%(14)C 从乙醇转移到脂肪酸。结果表明,在藻类培养基中添加适量的乙醇有利于生物柴油原料的生产和生物柴油的性质。