O'Shea J D, Rodgers R J, McCoy K, D'Occhio M J
Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
Acta Anat (Basel). 1990;138(2):154-65. doi: 10.1159/000146933.
Corpora lutea (CL) from cows on day 12 of the oestrous cycle were studied by electron microscopy to investigate whether, and how, different subpopulations of luteal cells can be identified in tissue sections. Tissues from 6 CL were examined, and nucleated profiles of luteal cells were classified as large, medium or small on the basis of their areas in electron micrographs. Cut-off points for area categories for large, medium and small-sized cells were based on diameters of greater than 25, 20-25 and less than 20 microns, respectively, if the measured areas were converted to a circular shape after correction for shrinkage. The only qualitative features which distinguished cells of large size from those of small size were the presence of clusters of secretory granules, and of exocytosis of these granules, in large cells only. However, these features were observed in only 59% of large cells, probably primarily due to sampling limitations in single sections. Other qualitative features which have been regarded as diagnostic of large or small luteal cells were observed in cells in all size categories. It was concluded that large and small luteal cells in the cyclic CL of the cow are distinguishable by their ultrastructural features. However, these data do not support the recent suggestion that the mid-cycle CL of the cow contain two subpopulations of large luteal cells in approximately equal numbers.
通过电子显微镜研究发情周期第12天奶牛的黄体（CL），以调查在组织切片中是否以及如何识别黄体细胞的不同亚群。检查了6个黄体的组织，并根据电子显微镜照片中黄体细胞有核轮廓的面积将其分类为大、中或小。如果将测量面积校正收缩后转换为圆形，大、中、小细胞面积类别的分界点分别基于直径大于25、20 - 25和小于20微米。区分大细胞和小细胞的唯一定性特征是大细胞中存在分泌颗粒簇以及这些颗粒的胞吐作用，然而，这些特征仅在59%的大细胞中观察到，可能主要是由于单切片中的取样限制。在所有大小类别的细胞中都观察到了其他被认为是大或小黄体细胞诊断特征的定性特征。得出的结论是，奶牛周期性黄体中的大、小黄体细胞可通过其超微结构特征区分。然而，这些数据不支持最近的观点，即奶牛周期中期的黄体包含数量大致相等的两个大黄体细胞亚群。
