使用双色同时激发的绿色荧光蛋白单分子显微镜。
Single-molecule microscopy of the green fluorescent protein using simultaneous two-color excitation.
机构信息
Department Chemie, Lehrstuhl Physikalische Chemie I, Ludwig-Maximilians Universität München, München, Germany.
出版信息
Chemphyschem. 2001 Jun 18;2(6):392-6. doi: 10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7.
In vivo microscopy of the Green Fluorescent Protein (GFP), the most important label in cell biology, with single-molecule sensitivity is hampered by an insufficient signal-to-noise ratio. A significant improvement is obtained with a novel two-color excitation technique. The picture clearly shows the increased brightness of GFP in in vitro single-molecule assays and in live-cell microscopy under two-color illumination (upper cell) as compared to normal illumination (lower cell).
体内显微镜观察绿色荧光蛋白（GFP），这是细胞生物学中最重要的标记物，具有单分子灵敏度，但由于信噪比不足而受到阻碍。一种新的双色激发技术可显著提高其灵敏度。与正常激发（下细胞）相比，该图清楚地显示了在体外单分子测定和双色激发下活细胞显微镜中 GFP 的亮度显著增加（上细胞）。
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