Division of Renal Diseases and Hypertension, Department of Medicine, University of Colorado, Denver, CO, USA.
Pediatr Nephrol. 2013 Sep;28(9):1803-12. doi: 10.1007/s00467-013-2498-4. Epub 2013 May 21.
Minimal change disease (MCD) is the most common cause of nephrotic syndrome in children and is associated with the expression of CD80 in podocytes and the increased excretion of CD80 in urine. We hypothesized that serum from patients with MCD might stimulate CD80 expression in cultured podocytes.
Sera and peripheral blood mononuclear cells (PBMCs) were collected from subjects with MCD in relapse and remission and from normal controls. Immortalized human podocytes were incubated with culture media containing patient sera or supernatants from patient and control PBMC cultures. CD80 expression was measured by quantitative PCR and western blot analysis.
Sera collected from patients with MCD in relapse, but not in remission, significantly increased CD80 expression (mean ± standard deviation: 1.8 ± 0.7 vs. 0.8 ± 0.2; p < 0.004) and CD80 protein secretion by podocytes (p < 0.05 between relapse and normal controls). No such CD80 increase was observed when podocytes were incubated with supernatants of PBMC cultures from patients in relapse.
Sera from MCD patients in relapse, but not in remission, stimulated CD80 expression in cultured podocytes. Identifying this factor in sera could provide insights into the pathogenesis of this disorder. No role in CD80 expression by podocytes was found for cytokines released by PBMCs.
微小病变性肾病（MCD）是儿童肾病综合征最常见的病因，与足细胞中 CD80 的表达和 CD80 在尿液中的排泄增加有关。我们假设 MCD 患者的血清可能会刺激培养的足细胞中 CD80 的表达。
从 MCD 复发和缓解期的患者和正常对照中收集血清和外周血单核细胞（PBMC）。将永生化的人足细胞与含有患者血清或患者和对照 PBMC 培养物上清液的培养基一起孵育。通过定量 PCR 和 Western blot 分析来测量 CD80 的表达。
来自 MCD 复发患者的血清，但不是缓解期患者的血清，显著增加了足细胞的 CD80 表达（平均值 ± 标准差：1.8 ± 0.7 与 0.8 ± 0.2；p < 0.004）和 CD80 蛋白分泌（复发与正常对照组之间 p < 0.05）。当将足细胞与来自复发患者的 PBMC 培养物上清液孵育时，未观察到这种 CD80 增加。
来自 MCD 复发患者的血清，但不是缓解期患者的血清，刺激了培养的足细胞中 CD80 的表达。在血清中鉴定出这种因子可以深入了解该疾病的发病机制。在 PBMC 释放的细胞因子对足细胞 CD80 表达中未发现作用。
