微小病变病复发患者的肾小球内皮细胞和足细胞可表达CD80。
Glomerular endothelial cells and podocytes can express CD80 in patients with minimal change disease during relapse.
作者信息
Cara-Fuentes Gabriel, Venkatareddy Madhusudan, Verma Rakesh, Segarra Alfons, Cleuren Audrey C, Martínez-Ramos Alfonso, Johnson Richard J, Garg Puneet
机构信息
Division of Pediatric Nephrology, Department of Pediatrics, University of Michigan, MSRB-2, Room 1574, 1500 E Medical Center Dr, Ann Arbor, MI, 48109, USA.
Division of Nephrology, Department of Internal Medicine, University of Michigan, Ann Arbor, USA.
出版信息
Pediatr Nephrol. 2020 Oct;35(10):1887-1896. doi: 10.1007/s00467-020-04541-3. Epub 2020 May 12.
BACKGROUND
Urinary CD80 has emerged as potential biomarker in idiopathic nephrotic syndrome (INS). However, its cellular source remains controversial. The aim of the study was to assess whether CD80 is truly expressed by glomerular cells in INS patients during relapse and in the LPS mouse model of podocyte injury.
METHODS
The presence of CD80 in glomeruli was evaluated by combining immunostaining, immunogold labeling, and in situ hybridization techniques.
RESULTS
CD80 was present along the surface of glomerular endothelial cells (GEC) and rarely in podocytes in six of nine minimal change disease (MCD) patients in relapse, two of eleven patients with focal segmental glomerulosclerosis in relapse, and absent in controls. In mice, CD80 was upregulated at mRNA and protein level in GEC and podocytes, in a similar pattern to that seen in MCD patients.
CONCLUSIONS
Glomerular endothelial cells and podocytes can express CD80 in patients with MCD during relapse. A better understanding of the role of CD80 in glomerular cells may provide further insights into the mechanisms of proteinuria in INS.
背景
尿CD80已成为特发性肾病综合征（INS）的潜在生物标志物。然而，其细胞来源仍存在争议。本研究的目的是评估在复发期间的INS患者以及足细胞损伤的脂多糖小鼠模型中，肾小球细胞是否真的表达CD80。
方法
通过结合免疫染色、免疫金标记和原位杂交技术评估肾小球中CD80的存在情况。
结果
在9例复发的微小病变病（MCD）患者中的6例、11例复发的局灶节段性肾小球硬化患者中的2例中，CD80存在于肾小球内皮细胞（GEC）表面，在足细胞中很少见，而在对照组中不存在。在小鼠中，GEC和足细胞中的CD80在mRNA和蛋白质水平上上调，其模式与MCD患者中所见相似。
结论
复发期间MCD患者的肾小球内皮细胞和足细胞可表达CD80。更好地了解CD80在肾小球细胞中的作用可能会为INS中蛋白尿的机制提供进一步的见解。
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