ER-α36 表达水平降低与 PCOS 患者子宫内膜增生的发生有关。
Lower expression of ER-α36 is associated with the development of endometrial hyperplasia in PCOS patients.
机构信息
Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, and Key Laboratory of Assisted Reproduction, Ministry of Education, and Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China.
出版信息
Histol Histopathol. 2013 Nov;28(11):1491-8. doi: 10.14670/HH-28.1491. Epub 2013 May 21.
OBJECTIVE
To explore the expression change of ER-α36 in endometria of PCOS patients.
METHODS
Proliferative endometria were collected and divided into three groups: CE group (n=30), proliferative endometria from control women; PCOSE group (n=30), proliferative endometria from PCOS patients; HPCOSE group (n=15), hyperplastic endometria from PCOS patients. The cellular localization of ER-α36 and ER-α66 was analyzed by immunohistochemistry, and expression of ER-α36 and ER-α66 was determined by immunohistochemistry and Real-Time Quantitative PCR. The correlation between serum hormone concentration and expression of ER-α36 and ER-α66 was analyzed.
RESULTS
ER-α36 was localized on the cell surface, cytoplasm and nucleus of glandular epithelial cells of both CE and PCOSE groups, mainly on the cell surface, and ER-α66 was localized on the nucleus. The protein and mRNA expression of ER-α36 was decreased from CE, PCOSE to HPCOSE group, while expression of ER-α66 showed no obvious difference among groups. The expression ratio of ER-α36 to ER-α66 of CE, PCOSE and HPCOSE group showed a decreasing tendency. The expression of ER-α36 and its expressive ratio to ER-α66 was negatively correlated with serum concentration of LH, LH/FSH, testosterone and androstenedione.
CONCLUSIONS
Lower expression of ER-α66 is associated with the development of endometrial hyperplasia in PCOS patients.
目的
探讨 ER-α36 在多囊卵巢综合征（PCOS）患者子宫内膜中的表达变化。
方法
收集增生期子宫内膜并分为三组：CE 组（n=30），来自对照组妇女的增生期子宫内膜；PCOSE 组（n=30），来自 PCOS 患者的增生期子宫内膜；HPCOSE 组（n=15），来自 PCOS 患者的增生性子宫内膜。通过免疫组织化学分析 ER-α36 和 ER-α66 的细胞定位，并通过免疫组织化学和实时定量 PCR 测定 ER-α36 和 ER-α66 的表达。分析血清激素浓度与 ER-α36 和 ER-α66 表达之间的相关性。
结果
ER-α36 定位于 CE 和 PCOSE 组的腺上皮细胞的细胞膜、细胞质和细胞核，主要位于细胞膜上，而 ER-α66 定位于细胞核。从 CE 到 HPCOSE 组，ER-α36 的蛋白和 mRNA 表达均降低，而 ER-α66 的表达在各组之间无明显差异。CE、PCOSE 和 HPCOSE 组 ER-α36 与 ER-α66 的表达比值呈下降趋势。CE、PCOSE 和 HPCOSE 组 ER-α36 的表达及其与 ER-α66 的表达比值与血清 LH、LH/FSH、睾酮和雄烯二酮浓度呈负相关。
结论
ER-α66 的低表达与 PCOS 患者子宫内膜增生的发生有关。
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