人类腮腺唾液中的脱氧核糖核酸酶。
Deoxyribonuclease in human parotid saliva.
作者信息
Zöllner E J, Klepsch D M, Zahn R K, Knepper R
出版信息
Enzyme. 1975;19(1):60-4. doi: 10.1159/000458972.
Deoxyribonuclease (EC 3.1.4.5, EC 3.1.4.6) activities in human parotid saliva were examined by microdisc electrophoresis. Three fractions, differing in their electrophoretic mobility and in their optimal incubation conditions, were characterized. The various enzyme activities were tested at pH 5.0 in 100 mmol with l-minus 1 Na-acetate buffer or at pH 7.04 in 100 mmol with l-minus 1 Tris-HCl-buffer in the presence of different combinations of MgCl(2), CaCl(2), EDTA, and Na(2)SO(4).
通过微量圆盘电泳检测人腮腺唾液中的脱氧核糖核酸酶（EC 3.1.4.5，EC 3.1.4.6）活性。鉴定出了三个在电泳迁移率和最佳孵育条件方面存在差异的组分。在含有不同组合的氯化镁、氯化钙、乙二胺四乙酸和硫酸钠的情况下，分别在pH 5.0的100 mmol/L乙酸钠缓冲液或pH 7.04的100 mmol/L Tris-HCl缓冲液中测试各种酶活性。
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