Department of Gastroenterology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China.
Mediators Inflamm. 2013;2013:617145. doi: 10.1155/2013/617145. Epub 2013 Apr 14.
Prohibitin, which can inhibit oxidative stress and mitochondrial dysfunction, has been shown to have significant anti-inflammatory activities. Here, we investigate the effects of altering prohibitin levels in affected tissues in the interleukin-10 knockout (IL-10KO) mouse model with intestinal fibrosis. The aim of this study is to investigate the effects of IL-10 on prohibitin and the role of prohibitin in intestinal fibrosis of murine colitis. After the mice were treated with IL-10, prohibitin expression and localization were evaluated in IL-10KO and wild-type (WT, 129/SvEv) mice. The colon tissue was then investigated and the potential pathogenic molecular mechanisms were further studied. Fluorescence-based quantitative polymerase chain reaction (FQ-PCR) and immunohistochemistry assays revealed a significant upregulation of prohibitin with IL-10 treatment. Furthermore, IL-10 decreases inflammatory cytokines and TGF-β1 in the IL-10KO model of Crohn's disease and demonstrates a promising trend in decreasing tissue fibrosis. In conclusion, we hypothesize that IL-10 treatment is associated with increased prohibitin and would decrease inflammation and fibrosis in an animal model of Crohn's disease. Interestingly, prohibitin may be a potential target for intestinal fibrosis associated with inflammatory bowel disease (IBD).
抑制素可以抑制氧化应激和线粒体功能障碍，具有显著的抗炎活性。在这里，我们研究了改变白细胞介素 10 敲除（IL-10KO）小鼠模型中受影响组织中抑制素水平对肠道纤维化的影响。本研究旨在研究白细胞介素 10 对抑制素的影响以及抑制素在鼠结肠炎肠道纤维化中的作用。在对小鼠进行白细胞介素 10 处理后，评估了白细胞介素 10KO 和野生型（WT，129/SvEv）小鼠中抑制素的表达和定位。然后研究了结肠组织，并进一步研究了潜在的致病分子机制。荧光定量聚合酶链反应（FQ-PCR）和免疫组织化学检测显示，白细胞介素 10 处理后抑制素显著上调。此外，白细胞介素 10 降低了克罗恩病 IL-10KO 模型中的炎症细胞因子和 TGF-β1，并显示出在降低组织纤维化方面有良好的趋势。总之，我们假设白细胞介素 10 治疗与抑制素的增加有关，并可在克罗恩病的动物模型中减轻炎症和纤维化。有趣的是，抑制素可能是与炎症性肠病（IBD）相关的肠道纤维化的一个潜在靶点。
