Cancer Cell Biology Program, Peter MacCallum Cancer Centre, East Melbourne, VIC, Australia.
Adv Exp Med Biol. 2013;786:353-68. doi: 10.1007/978-94-007-6621-1_19.
Adult stem cells reside in most parts of the body where high tissue turn-over is evident. However there are vastly different demands on the number of cells that might be produced and no better examples of each extreme are the neurogenic zones of the brain, and the crypt compartments of the intestines. From a perspective of understanding the function of the transcription factor Myb, we have explored the biology of stem cell niches in both these radically different tissues. Each tissue has remarkable features, provide different in vivo and in vitro options for manipulation and open up novel insights into damage responses and diseases like cancer. A variety of studies using mouse models, conditional and hypomorphic Myb mutants, radiation induced damage and primary in vitro assays have advanced our understanding of both stem cell niches and has revealed a previously unrecognised role for Myb in the regulation of stem cells.
成体干细胞存在于大多数组织中，这些组织的组织更新率很高。然而，可能产生的细胞数量的要求却有很大的不同，没有比这两个极端更能说明问题的例子了，一个是大脑的神经生成区，另一个是肠道的隐窝区。从理解转录因子 Myb 功能的角度来看，我们已经探索了这两种截然不同的组织中干细胞龛的生物学特性。每个组织都有显著的特点，为体内和体外的操作提供了不同的选择，并为损伤反应和癌症等疾病提供了新的见解。利用小鼠模型、条件性和低功能 Myb 突变体、辐射诱导损伤和原代体外检测等多种研究方法，提高了我们对干细胞龛的认识，并揭示了 Myb 在调节干细胞方面以前未被认识到的作用。
