Gemalmaz Halil Can, Bolukbasi Selcuk, Esen Erdinc, Erdogan Deniz, Gürgen Seren Gülsen, Bardakci Yesim
Department of Orthopedics and Traumatology, Gazi University School of Medicine, Ankara, Turkey.
Int J Artif Organs. 2013 May 17;36(5):341-9. doi: 10.5301/ijao.5000228. Epub 2013 May 21.
Periosteal adventitia is believed to consist of fibrous tissue without any regenerative potential. This theory results in the assumption that surgically stripped periosteum which is also adventitial has no bone regeneration potential. We decided to test whether the periosteal adventitia is osteoinductive and whether it is suitable for a commonly faced clinical situation in an animal model.
This study used 24 femurs from 12 rabbits, which were separated into 3 groups. Lateral femoral condylar cavitary defects were created with a 5 mm drill bit. In group I, the defects were left empty as the control. In group II, the defects were only filled with ceramic graft particles. In group III, the defects were filled with a mixture of ceramic graft particles and autogenous, adventitial, periosteal particles. All animals were sacrificed at the end of the 6th week and were evaluated histologically.
The microscopy of 3 different histologists suggested that group III had far superior healing when compared to the control group and group II. The statistical evaluation of the histomorphometrically gathered quantitative results revealed a meaningful increase in woven bone and a decrease in fibrous tissue in group III, confirming the histological analysis.
In this study we observed that the composite graft obtained by mixing ceramics and free adventitial periosteal grafts offers healing potential surpassing both the ceramic-only group as well as the control group. We conclude that adventitial periosteal graft greatly facilitates new bone formation.
骨膜外膜被认为由无任何再生潜力的纤维组织构成。这一理论导致一种假设，即手术剥离的同样为外膜性的骨膜没有骨再生潜力。我们决定在动物模型中测试骨膜外膜是否具有骨诱导性，以及它是否适用于一种常见的临床情况。
本研究使用了来自12只兔子的24根股骨，将其分为3组。用5毫米的钻头在股骨外侧髁制造腔洞性缺损。在第一组中，缺损处不做处理作为对照。在第二组中，缺损处仅填充陶瓷移植颗粒。在第三组中，缺损处填充陶瓷移植颗粒与自体、外膜性、骨膜颗粒的混合物。所有动物在第6周结束时处死，并进行组织学评估。
3位不同组织病理学家的显微镜检查表明，与对照组和第二组相比，第三组的愈合情况要好得多。对组织形态计量学收集的定量结果进行的统计学评估显示，第三组编织骨有显著增加，纤维组织减少，这证实了组织学分析。
在本研究中，我们观察到通过混合陶瓷和游离外膜性骨膜移植物获得的复合移植物具有超过仅使用陶瓷组和对照组的愈合潜力。我们得出结论，外膜性骨膜移植物极大地促进了新骨形成。
