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[基于响应面法的生防菌吡咯伯克霍尔德氏菌JK-SH007发酵优化]

[Optimization of biocontrol agent Burkholderia pyrrocinia strain JK-SH007 fermentation by response surface methodology].

作者信息

Li Hao, Ren Jiahong, Ye Jianren

机构信息

College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2013 Feb;29(2):243-6.

Abstract

In order to improve ferment efficiency of biocontrol agent Burkholderia pyrrocinia JK-SH007, the fermentation conditions of this strain were optimized. The optimal fermentation conditions were corn steep liquor (13.88 g/L) and glucose (3.37 g/L) by screening test, steepest ascent experiments and response surface analysis. The results showed that the cell density of JK-SH007 (1.18 x 10(9) CFU/mL) increased 1.35 times than before, and there was a 28.84% increase in antifungal activity.

摘要

为提高生防菌吡咯伯克霍尔德氏菌JK-SH007的发酵效率,对该菌株的发酵条件进行了优化。通过筛选试验、最陡爬坡试验和响应面分析,确定最佳发酵条件为玉米浆(13.88 g/L)和葡萄糖(3.37 g/L)。结果表明,JK-SH007的细胞密度(1.18×10⁹ CFU/mL)比之前提高了1.35倍,抑菌活性提高了28.84%。

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