Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.
Anal Chem. 2013 Jun 18;85(12):5617-21. doi: 10.1021/ac4009012. Epub 2013 May 24.
Here, we report the first use of resonance Raman scattering for the detection of miniaturized microscale arrays fabricated by dip-pen nanolithography. Antibody arrays for prostate-specific antigen (PSA) were printed, and a sandwich immunoassay was carried out. An enzyme-linked detection antibody was used to provide an insoluble and stable colored microdot in the recommended size range for microarray readers, which could be read with resonance Raman scattering. This gives quantitative detection as well as an improved detection limit and a larger dynamic range than that previously achieved by direct fluorescent detection methods. By Raman mapping across the arrayed area, the microdots were easily detected with very little background signal from surrounding areas. Levels of PSA as low as 25 pg/mL were detected using this method, which could be extended to a large number of useful biomarkers.
在这里，我们报告了共振拉曼散射在检测通过浸笔纳米光刻技术制造的微型微阵列中的首次应用。我们打印了用于前列腺特异性抗原 (PSA) 的抗体阵列，并进行了三明治免疫测定。酶联检测抗体用于提供在微阵列读取器推荐尺寸范围内的不溶性和稳定的有色微点，该微点可以通过共振拉曼散射进行读取。这不仅提供了定量检测，还改善了检测限和动态范围，优于以前的直接荧光检测方法。通过在阵列区域上的拉曼映射，很容易检测到微点，并且来自周围区域的背景信号非常少。使用这种方法可以检测到低至 25 pg/mL 的 PSA 水平，并且可以扩展到许多有用的生物标志物。
