Kawada T, Aoki N, Kawai R, Sugimoto E
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Cell Biol Int Rep. 1990 Jun;14(6):567-74. doi: 10.1016/0309-1651(90)91184-6.
We have developed a completely defined serum-free medium that supports the growth of Swiss 3T3-L1 fibroblasts to nearly the same extent as Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum. With ASF301 medium [former name, RITC 80-7; Yamane et al. Exp. Cell Res. 134, 470 (1981)], most of the 3T3-L1 cells survived for at least 10 days, but did not grow. ASF301 medium contains insulin and mouse-epidermal growth factor as growth factors, which are termed "progression factors". So we examined the effects of "competent factors" on the proliferation of 3T3-L1 preadipocytes. Growth in the medium supplemented with competent factors reached a confluent monolayer in 6-7 days. Furthermore, it was confirmed that 3T3-L1 cells grown in the serum-free medium retained the properties of differentiation into adipocytes. Our serum-free medium should be a useful tool for research on the growth and differentiation of 3T3-L1 preadipocytes.
我们已经开发出一种完全限定的无血清培养基，其支持瑞士3T3-L1成纤维细胞生长的程度与补充有10%胎牛血清的杜尔贝科改良伊格尔培养基几乎相同。使用ASF301培养基[原名，RITC 80-7；山根等人。《实验细胞研究》134, 470 (1981)]，大多数3T3-L1细胞存活了至少10天，但没有生长。ASF301培养基含有胰岛素和小鼠表皮生长因子作为生长因子，这些被称为“进展因子”。因此，我们研究了“感受态因子”对3T3-L1前脂肪细胞增殖的影响。在补充有感受态因子的培养基中生长的细胞在6 - 7天内达到汇合的单层。此外，证实了在无血清培养基中生长的3T3-L1细胞保留了分化为脂肪细胞的特性。我们的无血清培养基应该是研究3T3-L1前脂肪细胞生长和分化的有用工具。
