Soft Matter Materials Branch, Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson AFB, OH, USA.
Biofouling. 2013;29(6):601-15. doi: 10.1080/08927014.2013.783906. Epub 2013 May 22.
Microbial biofilms cause the deterioration of polymeric coatings such as polyurethanes (PUs). In many cases, microbes have been shown to use the PU as a nutrient source. The interaction between biofilms and nutritive substrata is complex, since both the medium and the substratum can provide nutrients that affect biofilm formation and biodeterioration. Historically, studies of PU biodeterioration have monitored the planktonic cells in the medium surrounding the material, not the biofilm. This study monitored planktonic and biofilm cell counts, and biofilm morphology, in long-term growth experiments conducted with Pseudomonas fluorescens under different nutrient conditions. Nutrients affected planktonic and biofilm cell numbers differently, and neither was representative of the system as a whole. Microscopic examination of the biofilm revealed the presence of intracellular storage granules in biofilms grown in M9 but not yeast extract salts medium. These granules are indicative of nutrient limitation and/or entry into stationary phase, which may impact the biodegradative capability of the biofilm.
微生物生物膜会导致聚合物涂层（如聚氨酯 (PU)）劣化。在许多情况下，已经证明微生物将 PU 用作营养源。生物膜和营养基质之间的相互作用很复杂，因为介质和基质都可以提供影响生物膜形成和生物降解的营养物质。从历史上看，对 PU 生物降解的研究监测的是材料周围介质中的浮游细胞，而不是生物膜。这项研究在不同营养条件下对荧光假单胞菌进行了长期生长实验，监测了浮游细胞和生物膜细胞的计数以及生物膜形态。营养物质对浮游细胞和生物膜细胞数量的影响不同，两者都不能代表整个系统。生物膜的显微镜检查显示，在 M9 中生长的生物膜中存在细胞内储存颗粒，但在酵母提取物盐培养基中则没有。这些颗粒表明存在营养限制和/或进入静止期，这可能会影响生物膜的生物降解能力。
