Sövényi J F, Yamamoto H, Fujimoto S, Kusuda R
Veterinary Medical Research Institute, Hungarian Academy of Science, Budapest.
Dev Comp Immunol. 1990 Spring;14(2):185-200. doi: 10.1016/0145-305x(90)90090-2.
Pronounced changes occur in the kidneys carp injected with extracellular product (ECP), lipopolysaccharide (LPS) of A. salmonicida subsp. salmonicida, Freund's complete adjuvant (FCA). ECP caused 50-70% decrease in macrophages and neutrophils, whereas blasts and lymphocyte-like cells increased FCA-elicited increased percentages of blasts and a left shift of neutrophils after LPS injection. After both ECP and FCA injections, the density of major leucocyte types and red blood cells (RBC) in the kidney decreased. LPS generally increased the density of leucocytes. Granulocytes were larger after LPS injection and smaller after FCA injection than control cells. Perhaps ECP eliminates those cells potentially capable of nonspecific defense and FCA stimulates their proliferation. LPS seemed to induce mainly polyclonal lymphocyte-like cell propagation and a reduced replenishment of other leucocytes. Challenge morbidity was 100% of ECP-injected groups, 54.3% of controls, 37.1% of LPS-injected fish and 11.4% of FCA-injected groups. Disease susceptibility may be correlated with availability and functional status of macrophages and neutrophils.
给鲤鱼注射细胞外产物（ECP）、杀鲑气单胞菌亚种杀鲑气单胞菌的脂多糖（LPS）、弗氏完全佐剂（FCA）后，肾脏会发生显著变化。ECP导致巨噬细胞和中性粒细胞减少50 - 70%，而母细胞和淋巴细胞样细胞增加；FCA引发母细胞百分比增加，LPS注射后中性粒细胞左移。注射ECP和FCA后，肾脏中主要白细胞类型和红细胞（RBC）的密度均降低。LPS通常会增加白细胞密度。LPS注射后粒细胞比对照细胞大，FCA注射后粒细胞比对照细胞小。也许ECP消除了那些具有非特异性防御能力的细胞，而FCA刺激了它们的增殖。LPS似乎主要诱导多克隆淋巴细胞样细胞增殖，并减少其他白细胞的补充。攻毒发病率在注射ECP的组中为100%，在对照组中为54.3%，在注射LPS的鱼中为37.1%，在注射FCA的组中为11.4%。疾病易感性可能与巨噬细胞和中性粒细胞的可用性和功能状态相关。
