Bose M, Maity C R, Ghosal S K, Majumdar G
Zoology Department, Burdwan University, India.
Folia Parasitol (Praha). 1990;37(2):151-6.
The mode of absorption, kinetics and mechanism of transportation of 14C--glucose in a digenetic trematode, Ceylonocotyle scoliocoelium were studied by culturing the trematodes under in vitro condition in Hank's saline supplemented with radioactive tracer. It was then followed separately by autoradiography and liquid scintillation. Autoradiographic study reveals that the maximum amount of glucose uptake occurs through cuticle whereas the role of intestinal canal is insignificant in this respect. Maximum cpm is recorded in the precipitate followed by TCA treatment. This is due to the presence of a protein-bound 14C-glucose which is assumed as the carrier protein. The inhibitor, phlorizin, used here presumably acts noncompetitively.
通过在补充有放射性示踪剂的汉克氏盐溶液中体外培养双殖吸虫锡兰颈盘吸虫，研究了14C-葡萄糖在该吸虫中的吸收方式、动力学及转运机制。然后分别采用放射自显影术和液体闪烁法进行追踪。放射自显影研究表明，葡萄糖的最大摄取量通过表皮发生，而在这方面肠道的作用微不足道。经三氯乙酸处理后的沉淀物中记录到最高每分钟计数。这是由于存在一种假定为载体蛋白的与蛋白质结合的14C-葡萄糖。此处使用的抑制剂根皮苷可能起非竞争性作用。
