Giboda M, Ditrich O, Vokurková-Vrchotová N
Institute of Parasitology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
Folia Parasitol (Praha). 1990;37(2):97-104.
A total of 2,883 foreign students at the age of 18-30 years were examined for amoebiasis after their arrival to Czechoslovakia. Stool examinations revealed the presence of Entamoeba histolytica in 112 of them (3.9%). Students from 38 countries were found to be infected with this parasite. In a set of 2,064 students from these countries E. histolytica prevalence in stool was 5.4%. There were greater differences in the prevalence between individual countries inside a geographical region than between individual geographical regions. The highest E. histolytica prevalence in stool was found in students from tropical and southern Africa (6.7% of 745 examined) and the lowest in students from South-eastern Asia (3.1% of 321 examined). In a simple cross-section study, antibodies against E. histolytica were detected by enzyme-linked immunosorbent assay (ELISA) in the sera of 1,001 persons. Antibodies were detected in 7.9% of students at the following titres: 1:200 in 4.5%, 1:600 in 1.5%, 1:1,800 in 1.9%. Antibodies occurred more frequently in students carrying E. histolytica cysts (X2 = 14.9). Titre of ELISA antibodies in patients with confirmed liver abscess was higher than 1:1,800. counterimmunoelectrophoresis (CIEP) test was used for serum examinations of patients who had been demonstrated by ELISA to be seropositive and of those carrying E. histolytica cysts. In a set of 170 patients CIEP antibodies were also more frequent in those carrying E. histolytica cysts (X2 = 26.95). A comparison of the results of ELISA and CIEP tests in the same patients revealed that CIEP antibodies were more dependent on the actual parasitization with E. histolytica than ELISA antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)
对抵达捷克斯洛伐克的2883名年龄在18至30岁之间的外国学生进行了阿米巴病检查。粪便检查发现其中112人（3.9%）感染了溶组织内阿米巴。发现来自38个国家的学生感染了这种寄生虫。在来自这些国家的2064名学生中，粪便中溶组织内阿米巴的患病率为5.4%。地理区域内各国之间的患病率差异大于各地理区域之间的差异。粪便中溶组织内阿米巴患病率最高的是来自热带和南部非洲的学生（745名受检学生中的6.7%），最低的是来自东南亚的学生（321名受检学生中的3.1%）。在一项简单的横断面研究中，通过酶联免疫吸附测定（ELISA）在1001人的血清中检测到了抗溶组织内阿米巴抗体。7.9%的学生检测到抗体，其滴度如下：1:200的占4.5%，1:600的占1.5%，1:1800的占1.9%。携带溶组织内阿米巴包囊的学生中抗体出现得更频繁（X2 = 14.9）。确诊为肝脓肿的患者ELISA抗体滴度高于1:1800。对流免疫电泳（CIEP）试验用于对ELISA检测为血清阳性的患者以及携带溶组织内阿米巴包囊的患者进行血清检查。在170名患者中，携带溶组织内阿米巴包囊的患者CIEP抗体也更频繁（X2 = 26.95）。对同一患者的ELISA和CIEP试验结果进行比较发现，CIEP抗体比ELISA抗体更依赖于实际的溶组织内阿米巴寄生情况。（摘要截于250字）
