Uchida M, Nishimura N, Misaki N, Ikarashi Y, Kobayashi M, Kawano O
Research and Development Division, Grelan Pharmaceutical Co., Ltd., Tokyo, Japan.
Nihon Yakurigaku Zasshi. 1990 Apr;95(4):185-90. doi: 10.1254/fpj.95.4_185.
The effects of anti-ulcer drugs and PGE2 on the proliferation of rat gastric cultured cells were investigated. To study the cell proliferation, the rate of incorporation of 3H-thymidine into the cultured cells was measured. ULTROSER G, corresponding to fetal bovine serum, was found to dose-dependently increase the incorporation of 3H-thymidine into the cultured cells at a dose range of 1-4%. The incorporation of 3H-thymidine was highest at 36 hr after the inoculation of cells. Therefore, the effect of the drugs on the proliferation was performed under the condition of 2% ULTROSER G and evaluated at 36 hr. Aldioxa, cetraxate HCl, cimetidine (10(-7)-10(-5) M) and PGE2 (10(-8)-10(-6) M) had no effect on the cell proliferation, while indomethacin (0.35-1.41 x 10(-3) M) inhibited the proliferation in a dose-dependent manner. Aldioxa, cetraxate HCl (10(-5) M) and PGE2 (10(-6), 10(-8) M) antagonized the inhibitory effect by indomethacin at a concentration of 0.5 x 10(-3) M, but cimetidine (10(-7)-10(-5) M) did not. From the above results, it was concluded that this method was useful for investigating the effect of drugs on the proliferation of gastric cultured cells.
研究了抗溃疡药物和前列腺素E2（PGE2）对大鼠胃培养细胞增殖的影响。为了研究细胞增殖，测量了3H-胸腺嘧啶核苷掺入培养细胞的速率。发现与胎牛血清相当的ULTROSER G在1-4%的剂量范围内能剂量依赖性地增加3H-胸腺嘧啶核苷掺入培养细胞的量。接种细胞后36小时，3H-胸腺嘧啶核苷的掺入量最高。因此，在2% ULTROSER G的条件下进行药物对增殖的作用研究，并在36小时时进行评估。阿尔迪奥克、盐酸西曲酸酯、西咪替丁（10^(-7)-10^(-5) M）和PGE2（10^(-8)-10^(-6) M）对细胞增殖无影响，而吲哚美辛（0.35-1.41×10^(-3) M）以剂量依赖性方式抑制增殖。阿尔迪奥克、盐酸西曲酸酯（10^(-5) M）和PGE2（10^(-6)、10^(-8) M）在浓度为0.5×10^(-3) M时可拮抗吲哚美辛的抑制作用，但西咪替丁（10^(-7)-10^(-5) M）则不能。根据上述结果，得出该方法可用于研究药物对胃培养细胞增殖影响的结论。
