Çoban Jale, Bingül Ilknur, Yesil-Mizrak Kubra, Dogru-Abbasoglu Semra, Oztezcan Serdar, Uysal Mujdat
Department of Biochemistry, Yeditepe University Medical Faculty, Kayısdagi, Istanbul,Turkey.
Curr Aging Sci. 2013 Jul;6(2):199-205. doi: 10.2174/18746098112059990011.
Oxidative stress plays an important role in aging. Effects of several antioxidants on age-related oxidative stress have been investigated. Carnosine (CAR) and betaine have antioxidant actions. The combination of CAR with vitamin E(CAR+E) increases its antioxidant efficiency. We investigated the effects of CAR+E and betaine treatments on oxidative and antioxidative status in liver, heart and brain tissues of aged rats. Experiments were carried out on young (5 months)and aged (22 months) male Wistar rats. Aged rats were given CAR (250 mg/kg; i.p.; 5 days per week) and vitamin E (200mg/kg; i.m.; twice per week) or betaine (1% w/v) for two months. Malondialdehyde (MDA) and diene conjugate (DC)levels and antioxidants were measured. MDA and DC levels were higher in tissues of aged rats than young rats. Glutathione(GSH) levels decreased in liver, but not heart and brain. There were no changes in vitamin E and vitamin C levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione transferase (GST) activities in tissues of aged rats. CAR+E treatment was observed to decrease MDA and DC levels in tissues of aged rats. However, betaine decreased only hepatic MDA and DC levels. Both CAR+E and betaine increased hepatic GSH and vitamin E levels, but these treatments did not affect antioxidant enzyme activities. These results suggest that CAR+E treatment seems to be useful to decrease oxidative stress in liver, heart and brain tissues, but betaine is only effective in liver tissue of aged rats.
氧化应激在衰老过程中起着重要作用。已经研究了几种抗氧化剂对与年龄相关的氧化应激的影响。肌肽（CAR）和甜菜碱具有抗氧化作用。CAR与维生素E联合使用（CAR+E）可提高其抗氧化效率。我们研究了CAR+E和甜菜碱处理对老年大鼠肝脏、心脏和脑组织氧化及抗氧化状态的影响。实验在年轻（5个月）和老年（22个月）雄性Wistar大鼠身上进行。老年大鼠每周5天腹腔注射CAR（250mg/kg），每周两次肌肉注射维生素E（200mg/kg）或给予1%（w/v）甜菜碱，持续两个月。检测丙二醛（MDA）、二烯共轭物（DC）水平及抗氧化剂。老年大鼠组织中的MDA和DC水平高于年轻大鼠。肝脏中的谷胱甘肽（GSH）水平下降，但心脏和脑组织中未下降。老年大鼠组织中的维生素E、维生素C水平以及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽转移酶（GST）活性没有变化。观察到CAR+E处理可降低老年大鼠组织中的MDA和DC水平。然而，甜菜碱仅降低肝脏中的MDA和DC水平。CAR+E和甜菜碱均提高了肝脏中的GSH和维生素E水平，但这些处理并未影响抗氧化酶活性。这些结果表明，CAR+E处理似乎有助于降低肝脏、心脏和脑组织中的氧化应激，但甜菜碱仅对老年大鼠的肝脏组织有效。
