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代表23种不同血清型的肺炎球菌分离株的体外生长情况。

In vitro growth of pneumococcal isolates representing 23 different serotypes.

作者信息

Slotved Hans-Christian, Satzke Catherine

机构信息

Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.

出版信息

BMC Res Notes. 2013 May 23;6:208. doi: 10.1186/1756-0500-6-208.

DOI:10.1186/1756-0500-6-208
PMID:23701729
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3664610/
Abstract

BACKGROUND

Although there are established methodologies for pneumococcal carriage studies, recent studies have extended these by conducting a culture amplification step prior to pneumococcal identification or serotyping. However, few data are available comparing the growth of different serotypes.

FINDINGS

We compared the growth of individual isolates representing 23 serotypes in serum broth, and Todd-Hewitt broth from four different manufacturers. Following overnight incubation of low inocula, there were differences in the final growth densities of individual isolates. These can be minimised with the use of optimal media.

CONCLUSIONS

These data caution against using broth culture amplification of nasopharyngeal samples for some applications.

摘要

背景

尽管已有用于肺炎球菌携带研究的既定方法，但近期研究通过在肺炎球菌鉴定或血清分型之前进行培养扩增步骤对这些方法进行了扩展。然而，比较不同血清型生长情况的数据很少。

研究结果

我们比较了代表23种血清型的单个分离株在血清肉汤以及来自四个不同制造商的托德-休伊特肉汤中的生长情况。低接种量过夜培养后，单个分离株的最终生长密度存在差异。使用最佳培养基可将这些差异降至最低。

结论

这些数据提醒在某些应用中要谨慎使用鼻咽样本的肉汤培养扩增法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c7/3664610/71129782b625/1756-0500-6-208-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c7/3664610/99f608a8aa7e/1756-0500-6-208-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c7/3664610/71129782b625/1756-0500-6-208-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c7/3664610/99f608a8aa7e/1756-0500-6-208-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c7/3664610/71129782b625/1756-0500-6-208-2.jpg

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