Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
Dev Comp Immunol. 2013 Oct;41(2):137-52. doi: 10.1016/j.dci.2013.05.006. Epub 2013 May 20.
In this study, we have identified 421 molecules across the vertebrate spectrum and propose a unified nomenclature for CXC chemokines in fish, amphibians and reptiles based on phylogenetic analysis. Expanding on earlier studies in teleost fish, lineage specific CXC chemokines that have no apparent homologues in mammals were confirmed. Furthermore, in addition to the two subgroups of the CXCL8 homologues known in teleost fish, a third group was identified (termed CXCL8_L3), as was a further subgroup of the fish CXC genes related to CXCL11. Expression of the CXC chemokines found in rainbow trout, Oncorhynchus mykiss, was studied in response to stimulation with inflammatory and antiviral cytokines, and bacterial. Tissue distribution analysis revealed distinct expression profiles for these trout CXC chemokines. Lastly three of the trout chemokines, including two novel fish specific CXC chemokines containing three pairs of cysteines, were produced as recombinant proteins and their effect on trout leucocyte migration studied. These molecules increased the relative expression of CD4 and MCSFR in migrated cells in an in vitro chemotaxis assay.
在这项研究中，我们在整个脊椎动物范围内鉴定了 421 种分子，并基于系统发育分析为鱼类、两栖动物和爬行动物的 CXC 趋化因子提出了统一的命名法。在对硬骨鱼类的早期研究基础上，我们证实了在哺乳动物中没有明显同源物的特定谱系 CXC 趋化因子。此外，除了在硬骨鱼类中已知的两种 CXCL8 同源物亚群外，还鉴定出了第三组（称为 CXCL8_L3），以及与 CXCL11 相关的鱼类 CXC 基因的另一个亚群。我们研究了虹鳟鱼（Oncorhynchus mykiss）中发现的 CXC 趋化因子对炎症和抗病毒细胞因子以及细菌刺激的反应，组织分布分析揭示了这些虹鳟鱼 CXC 趋化因子的独特表达谱。最后，我们制备了三种虹鳟鱼趋化因子（包括两种含有三对半胱氨酸的新型鱼类特异性 CXC 趋化因子）作为重组蛋白，并研究了它们对虹鳟鱼白细胞迁移的影响。这些分子在体外趋化实验中增加了迁移细胞中 CD4 和 MCSFR 的相对表达。
