Cameron A A, Plenderleith M B, Snow P J
Department of Anatomy, University of Queensland, Brisbane, Australia.
J Comp Neurol. 1990 Jul 8;297(2):201-18. doi: 10.1002/cne.902970204.
An analysis of Nissl stained sections of the spinal cord taken from four species of elasmobranch showed that seven distinct cytoarchitectonic laminae are present. These laminae are compared with laminae described previously in the spinal cord of other vertebrates. The distribution of immunoreactivity to serotonin, substance P, somatostatin, calcitonin gene-related peptide, neuropeptide Y, and bombesin was determined in the brown stringray (Dasyatis fluviorum), the eagle ray (Aetobatis narinari), the shovelnose ray (Rhinobatis battilum), and the black-tip shark (Carcharhinus melanopterus). In all species, dense immunoreactivity to most substances tested was found in the outer part of the substantia gelatinosa. Many fibres and varicosities immunoreactive to substance P, calcitonin gene-related peptide, and bombesin were found in this region and smaller numbers of fibres were found in the nucleus proprius. Immunoreactivity to somatostatin consisted of coarse fibre bundles that entered the dorsal horn at the nucleus proprius and radiated dorsally to the substantia gelatinosa. Axons and varicosities immunoreactive to serotonin and neuropeptide Y were found in all regions of the dorsal horn but were concentrated in the outer part of the substantia gelatinosa. The distribution of immunoreactivity to met-enkephalin in the shovelnose ray was concentrated in the lateral third of the substantia gelatinosa and to a lesser extent in the nucleus proprius. The distribution of these substances is compared with that described in other vertebrates. Although the sensory information reaching the elasmobranch spinal cord is limited, compared with that of mammalian species, the distribution of these neuroactive factors in the dorsal horn of the two groups is strikingly similar.
对取自四种软骨鱼类的脊髓尼氏染色切片进行分析显示，存在七个不同的细胞构筑层。将这些层与先前在其他脊椎动物脊髓中描述的层进行比较。测定了棕色黄貂鱼（Dasyatis fluviorum）、鹰魟（Aetobatis narinari）、铲鼻魟（Rhinobatis battilum）和黑鳍鲨（Carcharhinus melanopterus）中血清素、P物质、生长抑素、降钙素基因相关肽、神经肽Y和蛙皮素的免疫反应分布。在所有物种中，在胶状质外部发现了对大多数测试物质的密集免疫反应。在该区域发现了许多对P物质、降钙素基因相关肽和蛙皮素免疫反应的纤维和曲张体，在固有核中发现的纤维数量较少。对生长抑素的免疫反应由粗纤维束组成，这些纤维束在固有核处进入背角并向背侧辐射至胶状质。对血清素和神经肽Y免疫反应的轴突和曲张体在背角的所有区域都有发现，但集中在胶状质的外部。铲鼻魟中甲硫氨酸脑啡肽的免疫反应分布集中在胶状质外侧三分之一处，在固有核中的程度较小。将这些物质的分布与其他脊椎动物中描述的分布进行比较。尽管与哺乳动物相比，到达软骨鱼类脊髓的感觉信息有限，但两组背角中这些神经活性因子的分布惊人地相似。
