Saeidi Z, Vatandoost H, Akhavan A A, Yaghoobi-Ershadi M R, Rassi Y, Arandian M H, Jafari R
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Vector Borne Dis. 2013 Mar;50(1):57-61.
BACKGROUND & OBJECTIVES: Phlebotomine sandflies (Diptera: Psychodidae) play main role in the transmission of different forms of leishmaniasis in the world. Phlebotomus papatasi is the main vector of zoonotic cutaneous leishmaniasis (ZCL) in Iran. There are several control measures for vector control using different classes of insecticides. The aim of this study was to breed the sandflies which were collected from a hyperendemic focus of the disease in central Iran in the laboratory condition and to determine its baseline susceptibility to commonly used insecticides.
Sandflies were collected from the field and were reared in insectary. Susceptibility tests were carried out on their generation. Baseline susceptibility of sandflies to DDT and pyrethroids was evaluated based on LT50 values. A total of 1305 specimens were tested using different time intervals. The LT50 and LT90 values were measured according to the WHO standard tests. The results were plotted using probit analysis and regression lines.
The results against female sandflies revealed the LT50 values of 1312.66, 253.66, 36.04, 9.38 and 6 sec to DDT (4%), permethrin (0.75%), deltamethrin (0.1%), cyfluthrin (0.15%) and lambda-cyhalothrin (0.05%), respectively. The figures for male sandflies were 1200.97, 310.10, 18.63, 6.08 and 0.77 sec respectively to the above insecticides.
The results of this study could help to provide a clue for implementation of currently used insecticides. Furthermore, a specific guideline is needed for monitoring and evaluation of insecticide susceptibility test against sandflies.
白蛉（双翅目：毛蠓科）在全球不同形式利什曼病的传播中起主要作用。巴氏白蛉是伊朗动物源性皮肤利什曼病（ZCL）的主要传播媒介。使用不同种类的杀虫剂有多种病媒控制措施。本研究的目的是在实验室条件下饲养从伊朗中部该病高度流行地区采集的白蛉，并确定其对常用杀虫剂的基线敏感性。
从野外采集白蛉并在昆虫饲养室饲养。对其后代进行敏感性测试。根据半数致死时间（LT50）值评估白蛉对滴滴涕和拟除虫菊酯的基线敏感性。使用不同的时间间隔对总共1305个样本进行测试。根据世界卫生组织标准测试测量LT50和LT90值。使用概率分析和回归线绘制结果。
针对雌性白蛉的结果显示，对4%滴滴涕、0.75%氯菊酯、0.1%溴氰菊酯、0.15%氟氯氰菊酯和0.05%高效氯氟氰菊酯的LT50值分别为1312.66、253.66、36.04、9.38和6秒。雄性白蛉对上述杀虫剂的相应数字分别为1200.97、310.10、18.63、6.08和0.77秒。
本研究结果有助于为当前使用的杀虫剂的实施提供线索。此外，需要一个特定的指南来监测和评估针对白蛉的杀虫剂敏感性测试。
