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1
In-vivo expansion of autologous limbal stem cell using simple limbal epithelial transplantation for treatment of limbal stem cell deficiency.采用简单的角膜缘上皮移植术进行自体角膜缘干细胞的体内扩增以治疗角膜缘干细胞缺乏症。
BMJ Case Rep. 2013 May 22;2013:bcr2013009247. doi: 10.1136/bcr-2013-009247.
2
Cultivated limbal epithelial transplantation in children with ocular surface burns.培养的角膜缘上皮移植治疗儿童眼表烧伤。
JAMA Ophthalmol. 2013 Jun;131(6):731-6. doi: 10.1001/jamaophthalmol.2013.2308.
3
Successful autologous simple limbal epithelial transplantation (SLET) in previously failed paediatric limbal transplantation for ocular surface burns.在先前失败的小儿眼表烧伤角膜缘移植术中成功进行自体单纯角膜缘上皮移植(SLET)。
BMJ Case Rep. 2013 May 10;2013:bcr2013009888. doi: 10.1136/bcr-2013-009888.
4
Simple Limbal Epithelial Transplantation: Long-Term Clinical Outcomes in 125 Cases of Unilateral Chronic Ocular Surface Burns.单纯性角膜缘上皮移植术治疗单侧慢性眼表烧伤 125 例的长期临床疗效
Ophthalmology. 2016 May;123(5):1000-10. doi: 10.1016/j.ophtha.2015.12.042. Epub 2016 Feb 17.
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Amniotic membrane transplantation for partial and total limbal stem cell deficiency secondary to chemical burn.羊膜移植治疗化学烧伤继发的部分和完全性角膜缘干细胞缺乏症。
Ophthalmology. 2003 Mar;110(3):466-73. doi: 10.1016/s0161-6420(02)01888-2.
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Clinical outcomes of repeat autologous cultivated limbal epithelial transplantation for ocular surface burns.重复自体培养角膜缘上皮移植治疗眼表烧伤的临床疗效。
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Molten metal ocular burn: long-term outcome using simple limbal epithelial transplantation.熔融金属性眼烧伤:采用简单的角膜缘上皮移植术的长期疗效
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Early Results of Penetrating Keratoplasty in Patients With Unilateral Chemical Injury After Simple Limbal Epithelial Transplantation.单纯角膜缘上皮移植术后单侧化学伤患者穿透性角膜移植术的早期结果
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Simple limbal epithelial transplantation (SLET) in failed cultivated limbal epithelial transplantation (CLET) for unilateral chronic ocular burns.在单侧慢性眼部烧伤中,对于失败的培养角膜缘上皮移植(CLET),行单纯性角膜缘上皮移植(SLET)。
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Simple limbal epithelial transplantation: a review on current approach and future directions.简单性角膜缘上皮移植:当前方法和未来方向的综述。
Surv Ophthalmol. 2018 Nov-Dec;63(6):869-874. doi: 10.1016/j.survophthal.2018.05.003. Epub 2018 May 22.

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Simple limbal epithelial transplantation: Current status and future perspectives.单纯性角膜缘上皮移植:现状与未来展望。
Stem Cells Transl Med. 2020 Mar;9(3):316-327. doi: 10.1002/sctm.19-0203. Epub 2019 Dec 4.
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Twenty years of limbal epithelial therapy: an update on managing limbal stem cell deficiency.二十年的角膜缘上皮治疗:角膜缘干细胞缺乏症管理的最新进展
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Optimizing the role of limbal explant size and source in determining the outcomes of limbal transplantation: An in vitro study.优化角膜缘外植体大小和来源在确定角膜缘移植结果中的作用:一项体外研究。
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Simple Limbal Epithelial Transplantation in Acid Injury and Severe Dry Eye.单纯角膜缘上皮移植治疗酸烧伤及重度干眼
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Molten metal ocular burn: long-term outcome using simple limbal epithelial transplantation.熔融金属性眼烧伤:采用简单的角膜缘上皮移植术的长期疗效
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Construction of corneal epithelium with human amniotic epithelial cells and repair of limbal deficiency in rabbit models.用人羊膜上皮细胞构建角膜上皮并修复兔模型中的角膜缘缺损
Hum Cell. 2015 Jan;28(1):22-36. doi: 10.1007/s13577-014-0099-6. Epub 2014 Aug 19.
8
Keratinocyte growth factor-2 and autologous serum potentiate the regenerative effect of mesenchymal stem cells in cornea damage in rats.角质形成细胞生长因子-2和自体血清增强间充质干细胞对大鼠角膜损伤的再生作用。
Int J Ophthalmol. 2014 Apr 18;7(2):211-9. doi: 10.3980/j.issn.2222-3959.2014.02.05. eCollection 2014.
9
Transforming ocular surface stem cell research into successful clinical practice.将眼表干细胞研究转化为成功的临床实践。
Indian J Ophthalmol. 2014 Jan;62(1):29-40. doi: 10.4103/0301-4738.126173.

本文引用的文献

1
Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency.单纯性角膜缘上皮移植术(SLET):一种治疗单侧角膜缘干细胞缺陷的新型手术技术。
Br J Ophthalmol. 2012 Jul;96(7):931-4. doi: 10.1136/bjophthalmol-2011-301164. Epub 2012 Feb 10.
2
Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study.无饲养层自体培养角膜缘上皮移植的临床疗效:一项 10 年研究。
Br J Ophthalmol. 2011 Nov;95(11):1525-9. doi: 10.1136/bjophthalmol-2011-300352. Epub 2011 Sep 2.
3
Techniques for culture and assessment of limbal stem cell grafts.培养和评估角膜缘干细胞移植物的技术。
Ocul Surf. 2010 Jul;8(3):146-53. doi: 10.1016/s1542-0124(12)70225-2.
4
Limbal stem-cell therapy and long-term corneal regeneration.角膜缘干细胞治疗与长期角膜再生。
N Engl J Med. 2010 Jul 8;363(2):147-55. doi: 10.1056/NEJMoa0905955. Epub 2010 Jun 23.
5
Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium.用自体培养角膜上皮长期修复受损角膜表面。
Lancet. 1997 Apr 5;349(9057):990-3. doi: 10.1016/S0140-6736(96)11188-0.
6
Limbal autograft transplantation for ocular surface disorders.角膜缘自体移植治疗眼表疾病。
Ophthalmology. 1989 May;96(5):709-22; discussion 722-3. doi: 10.1016/s0161-6420(89)32833-8.

采用简单的角膜缘上皮移植术进行自体角膜缘干细胞的体内扩增以治疗角膜缘干细胞缺乏症。

In-vivo expansion of autologous limbal stem cell using simple limbal epithelial transplantation for treatment of limbal stem cell deficiency.

作者信息

Lal Ikeda, Panchal Bhavik Uttam, Basu Sayan, Sangwan Virender S

机构信息

Cornea and Anterior Segment Service, LV Prasad Eye Institute, Kallam Anji Reddy Campus, L V Prasad Marg, Banjara Hills, Hyderabad, Andhra Pradesh, India.

出版信息

BMJ Case Rep. 2013 May 22;2013:bcr2013009247. doi: 10.1136/bcr-2013-009247.

DOI:10.1136/bcr-2013-009247
PMID:23704435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3669860/
Abstract

A 20-year-old man from Bangladesh suffered accidental alkali injury to his right eye in May 2010 leading to total limbal stem cell deficiency. An amniotic membrane graft was performed 5 days after the accident and the patient presented to our institute 6 months later. On ocular examination, his best corrected visual acuity (BCVA) was 20/50 with a 360° pannus at the periphery and central area was spared but had stromal scarring. He underwent simple limbal epithelial transplantation (SLET) taking a limbal biopsy from his left eye and was prescribed steroid and antibiotic eye drops postoperatively as per the standard regimen. At 2 year follow-up, the patient's ocular surface is stable with improvement in  BCVA to 20/25 post-SLET.

摘要

一名来自孟加拉国的20岁男子于2010年5月右眼意外受到碱性物质损伤,导致全角膜缘干细胞缺乏。事故发生5天后进行了羊膜移植,6个月后患者前来我院就诊。眼部检查时,其最佳矫正视力(BCVA)为20/50,周边有360°血管翳,中央区域未受累但有基质瘢痕形成。他接受了简单的角膜缘上皮移植(SLET),取自左眼的角膜缘活检组织,术后按照标准方案给予类固醇和抗生素眼药水。在2年的随访中,患者的眼表稳定,SLET术后BCVA提高到20/25。