Recombinant Gene Products Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
J Nanobiotechnology. 2013 May 25;11:15. doi: 10.1186/1477-3155-11-15.
Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.
We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.
The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.
VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
登革热是当今最重要的虫媒病毒病。需要新的工具来有效解决登革热问题。病毒样颗粒（VLP）为开发具有潜在生物医学应用的工具提供了多功能的纳米级平台。从一种潜在有用的登革热特异性工具的角度来看，具有血清型特异性、宿主受体识别和诱导体外中和抗体能力的登革病毒包膜蛋白结构域 III（EDIII）是一个有吸引力的候选物。
我们开发了一种策略，以两种形式共表达和共纯化乙型肝炎病毒表面（S）抗原：独立表达和融合表达 EDIII。我们对其进行了物理和功能表征。
两种形式的 S 抗原都能组装成 VLP。这些 VLP 展示功能性可及的 EDIII 的能力取决于两种 S 抗原形式的相对水平。含有融合和非融合成分的 1:4 摩尔比的嵌合 VLP 显示出最大的功能能力。
武装有 EDIII 的 VLP 可能在诊断、治疗和预防应用中具有潜在的用途。
