人胎盘核糖体18S rRNA上mRNA结合中心区域一个位点的鉴定。
Identification of a site on 18 S rRNA of human placenta ribosomes in the region of the mRNA binding center.
作者信息
Graifer D M, Zenkova M A, Malygin A A, Mamaev S V, Mundus D A, Karpova G G
机构信息
Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of the USSR, Novosibirsk.
出版信息
J Mol Biol. 1990 Jul 5;214(1):121-8. doi: 10.1016/0022-2836(90)90151-B.
The affinity labeling of human placenta 80 S ribosomes by 4-(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphamide of hexauridylate was studied. This mRNA analog has normal coding properties because its binding to placenta ribosomes significantly increases in the presence of cognate tRNAPhe. Incubation of the mRNA analog in the complex with the ribosomes and Phe-tRNAPhe leads to its covalent attachment exclusively to the small subunit (mainly to 18 S rRNA). The site of the reaction has been identified by hybridization experiments to be located within positions 975 to 1055 of 18 S rRNA. The identified fragment is located in a highly conserved part of the small subunit rRNA domain II.
研究了六聚尿苷酸的4-(N-2-氯乙基-N-甲基氨基)苄基-5'-磷酸酰胺对人胎盘80S核糖体的亲和标记。这种mRNA类似物具有正常的编码特性，因为在同源苯丙氨酰tRNA存在时，它与胎盘核糖体的结合显著增加。将该mRNA类似物与核糖体和苯丙氨酰tRNA形成复合物进行温育，结果显示它仅共价连接到小亚基（主要是18S rRNA）上。通过杂交实验确定反应位点位于18S rRNA的975至1055位之间。所确定的片段位于小亚基rRNA结构域II的高度保守部分。
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