Castillo-Velázquez U, Gomez-Flores R, Tamez-Guerra R, Tamez-Guerra P, Rodríguez-Padilla C
Universidad Autónoma de Nuevo León, Departamento de Microbiología e Inmunología, Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Av. Pedro de Alba S/N, Cd. Universitaria, C.P. 66451 San Nicolás de los Garza, Nuevo León, Mexico.
Vet Immunol Immunopathol. 2013 Jul 15;154(1-2):8-16. doi: 10.1016/j.vetimm.2013.04.010. Epub 2013 May 6.
It is known that macrophages from naturally resistant animals possess a strong immune response against bovine tuberculosis to control mycobacterial infections. In the present study, the macrophage phagocytic activity, intracellular bacterial survival, and cytokine gene expression induced by classical and alternative activators against Mycobacterium bovis in naturally resistant or susceptible bovines, were evaluated. Animals were classified as naturally resistant or susceptible based on the capacity of their macrophages to allow M. bovis (BCG) growth. Peripheral blood macrophages from naturally resistant and susceptible animals were activated by classical and alternative stimuli and challenged with either non-pathogenic M. bovis BCG strain or pathogenic 9926 strain. Naturally resistant animals showed the highest phagocytosis index and microbial control after classical and alternative stimuli, being this response higher against the strain 9926 than the non-virulent strain. In addition, the response of macrophages activated by the classical pathway was higher than that under the alternative activation against both types of strains. Furthermore, classical pathway-activated macrophages derived from naturally resistant animals expressed higher levels of the pro-inflammatory markers iNOS, IL-1β, TNF-α, MIP-1 and MIP-3, and the anti-inflammatory markers ARGII and TGF-b, particularly to BCG. The results of this study showed that macrophages from naturally resistant animals produced stronger pro-inflammatory responses than those from susceptible ones to signals provided by classical pathway activators. Its role in innate immunity against M. bovis is yet to be determined.
已知来自天然抗性动物的巨噬细胞对牛结核病具有强大的免疫反应，以控制分枝杆菌感染。在本研究中，评估了天然抗性或易感牛中经典和替代激活剂诱导的巨噬细胞吞噬活性、细胞内细菌存活以及针对牛分枝杆菌的细胞因子基因表达。根据巨噬细胞允许牛分枝杆菌（卡介苗）生长的能力，将动物分为天然抗性或易感。来自天然抗性和易感动物的外周血巨噬细胞通过经典和替代刺激进行激活，并用非致病性牛分枝杆菌卡介苗菌株或致病性9926菌株进行攻击。天然抗性动物在经典和替代刺激后表现出最高的吞噬指数和微生物控制能力，对9926菌株的这种反应高于无毒力菌株。此外，经典途径激活的巨噬细胞对两种菌株的反应均高于替代激活下的反应。此外，来自天然抗性动物的经典途径激活的巨噬细胞表达更高水平的促炎标志物iNOS、IL-1β、TNF-α、MIP-1和MIP-3，以及抗炎标志物ARGII和TGF-b，尤其是对卡介苗。本研究结果表明，来自天然抗性动物的巨噬细胞对经典途径激活剂提供的信号产生的促炎反应比易感动物的更强。其在针对牛分枝杆菌的固有免疫中的作用尚待确定。
