Elizondo-Luevano Joel H, Quintanilla-Licea Ramiro, Monroy-García Imelda N, Kačániová Miroslava, Castillo-Velázquez Uziel, Bazaldúa-Rodríguez Aldo F, Garza-Vega Lourdes M, Torres-Hernández Ángel D, Chávez-Montes Abelardo
Department of Chemistry, Facultad de Ciencias Biológicas (FCB), Universidad Autónoma de Nuevo León (UANL), Ciudad Universitaria, San Nicolás de los Garza 66455, Nuevo León, Mexico.
Department of Chemical and Biochemical Engineering, Instituto Tecnológico de Los Mochis, Tecnológico Nacional de México (ITLM-TecNM), Juan de Dios Bátiz y 20 de Noviembre, Los Mochis 81259, Sinaloa, Mexico.
Plants (Basel). 2024 May 15;13(10):1374. doi: 10.3390/plants13101374.
L. has been used in traditional Mexican medicine. Among its bioactive constituents, berberine (BER) has garnered attention for its cytotoxic properties against different tumor cell lines. This study investigates the in vitro toxicity against HEP-G2 (human hepatocellular carcinoma) and murine lymphoma (L5178Y-R) cells using the MTT assay of the methanol extract (AmexM), sub-partitions of , and BER. Selectivity indices (SIs) were determined by comparing their cytotoxic effects on VERO (monkey kidney epithelial) and PBMC (human peripheral blood mononuclear) non-tumoral cells. Additionally, the anti-hemolytic effect of these treatments was assessed using the AAPH method. The treatment with the most promising activity against tumor cells and anti-hemolytic efficacy underwent further evaluation for toxicity in and antioxidant activities using DPPH, ABTS, and FRAP assays. BER demonstrated an IC = 56.86 µg/mL in HEP-G2 cells and IC < 5.0 µg/mL in L5178Y-R cells, with SI values of 15.97 and >5.40 in VERO and PBMC cells, respectively. No significant hemolytic effects were observed, although AmexM and BER exhibited the highest anti-hemolytic activity. BER also demonstrated superior antioxidant efficacy, with lower toxicity in nauplii compared to the control. Additionally, BER significantly attenuated nitric oxide production. This study highlights the antiproliferative effects of , particularly BER, against HEP-G2 and L5178Y-R tumor cell lines, along with its selectivity towards normal cells. Furthermore, its anti-hemolytic and antioxidant potentials were demonstrated, suggesting that BER is a promising candidate for potent chemotherapeutic agents.
L.已被用于墨西哥传统医学。在其生物活性成分中，黄连素（BER）因其对不同肿瘤细胞系的细胞毒性特性而受到关注。本研究使用甲醇提取物（AmexM）、其亚组分以及BER的MTT法，研究了对HEP-G2（人肝癌细胞）和鼠淋巴瘤（L5178Y-R）细胞的体外毒性。通过比较它们对VERO（猴肾上皮细胞）和PBMC（人外周血单核细胞）非肿瘤细胞的细胞毒性作用来确定选择性指数（SIs）。此外，使用AAPH方法评估了这些处理的抗溶血作用。对具有最有前景的肿瘤细胞活性和抗溶血功效的处理进行了进一步评估，使用DPPH、ABTS和FRAP测定法评估其在[具体生物]中的毒性和抗氧化活性。BER在HEP-G2细胞中的IC50 = 56.86 µg/mL，在L5178Y-R细胞中的IC50 < 5.0 µg/mL，在VERO和PBMC细胞中的SI值分别为15.97和>5.40。尽管AmexM和BER表现出最高的抗溶血活性，但未观察到明显的溶血作用。BER还表现出优异的抗氧化功效，与对照组相比，在[具体生物]无节幼体中的毒性较低。此外，BER显著减弱了一氧化氮的产生。本研究突出了[提取物名称]，特别是BER，对HEP-G2和L5178Y-R肿瘤细胞系的抗增殖作用，以及其对正常细胞的选择性。此外，还证明了其抗溶血和抗氧化潜力，表明BER是一种有前途的强效化疗药物候选物。
