Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Vaccine. 2013 Jul 11;31(32):3250-6. doi: 10.1016/j.vaccine.2013.05.047. Epub 2013 May 23.
Helicobacter suis causes gastric lesions in pigs and humans. This study aimed to evaluate the protective efficacy of immunization with combinations of the H. suis urease subunit B (UreB) and γ-glutamyl transpeptidase (GGT), both recombinantly expressed in Escherichia coli (rUreB and rGGT, respectively). Mice were intranasally immunized with rUreB, rGGT or a combination of both proteins, administered simultaneously or sequentially. Control groups consisted of non-immunized and non-challenged mice (negative controls), sham-immunized and H. suis-challenged mice (sham-immunized controls), and finally, H. suis whole-cell lysate-immunized and H. suis challenged mice. Cholera toxin was used as mucosal adjuvant. All immunizations induced a significant reduction of gastric H. suis colonization, which was least pronounced in the groups immunized with rGGT and rUreB only. Consecutive immunization with rGGT followed by rUreB and immunization with the bivalent vaccine improved the protective efficacy compared to immunization with single proteins, with a complete clearance of infection observed in 50% of the animals. Immunization with whole-cell lysate induced a similar reduction of gastric bacterial colonization compared to rGGT and rUreB in combinations. Gastric lesions, however, were less pronounced in mice immunized with combinations of rUreB and rGGT compared to mice immunized with whole-cell lysate. In conclusion, vaccination with a combination of rGGT and rUreB protected mice against a subsequent H. suis infection and was not associated with severe post-vaccination gastric inflammation, indicating that it may be a promising method for control of H. suis infections.
猪和人类的胃病变由猪幽门螺杆菌引起。本研究旨在评估免疫接种重组大肠杆菌表达的尿素酶亚单位 B（rUreB）和γ-谷氨酰转肽酶（rGGT）的组合对猪幽门螺杆菌的保护效力，这两种蛋白分别为（UreB）和γ-谷氨酰转肽酶（GGT）。通过鼻腔内免疫接种 rUreB、rGGT 或这两种蛋白的混合物，同时或顺序给予这些蛋白。对照组由未免疫和未 challenged 的小鼠（阴性对照）、假免疫和 H. suis-challenged 小鼠（假免疫对照）以及最后 H. suis 全细胞裂解物免疫和 H. suis-challenged 小鼠组成。霍乱毒素被用作粘膜佐剂。所有免疫接种均显著降低了胃幽门螺杆菌的定植，其中仅用 rGGT 和 rUreB 免疫接种的组效果最差。与单独使用 rGGT 或 rUreB 免疫接种相比，用 rGGT 进行连续免疫接种然后用双价疫苗进行免疫接种可提高保护效力，在 50%的动物中观察到感染完全清除。与单独使用 rGGT 和 rUreB 相比，用全细胞裂解物免疫接种可降低胃细菌定植率。然而，与用全细胞裂解物免疫接种的小鼠相比，用 rUreB 和 rGGT 组合免疫接种的小鼠的胃病变程度较轻。总之，用 rGGT 和 rUreB 的组合进行疫苗接种可保护小鼠免受随后的 H. suis 感染，并且与接种后的严重胃部炎症无关，表明它可能是控制 H. suis 感染的一种有前途的方法。
