Department of Psychiatry and Behavioral Sciences, Center for Sleep Sciences, Beckman Center, Stanford University, Palo Alto, CA 94305, USA.
Methods. 2013 Aug 15;62(3):292-303. doi: 10.1016/j.ymeth.2013.05.002. Epub 2013 May 24.
Video processing is increasingly becoming a standard procedure in zebrafish behavior investigations as it enables higher research throughput and new or better measures. This trend, fostered by the ever increasing performance-to-price ratio of the required recording and processing equipment, should be expected to continue in the foreseeable future, with video-processing based methods permeating more and more experiments and, as a result, expanding the very role of behavioral studies in zebrafish research. To assess whether the routine video tracking of zebrafish larvae directly in the Petri dish is a capability that can be expected in the near future, the key processing concepts are discussed and illustrated on published zebrafish studies when available or other animals when not.
视频处理在斑马鱼行为研究中越来越成为标准程序，因为它可以提高研究效率并提供新的或更好的测量方法。这种趋势受到所需记录和处理设备的性价比不断提高的推动，预计在可预见的未来还将继续，基于视频处理的方法将渗透到越来越多的实验中，从而扩展了行为研究在斑马鱼研究中的作用。为了评估在不久的将来是否可以直接在培养皿中对斑马鱼幼虫进行常规视频跟踪，讨论了关键的处理概念，并在可用时根据已发表的斑马鱼研究或其他动物的研究进行了说明。
