Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
Vaccine. 2013 Jul 11;31(32):3295-9. doi: 10.1016/j.vaccine.2013.04.061. Epub 2013 May 23.
Clostridium perfringens is an anaerobic spore-forming, pathogenic bacterium that is responsible for severe diseases in humans and livestock. In the present study, an epsilon-beta fusion toxin was expressed as a soluble protein in E. coli and the recombinant cell lysate was used for immunization studies in mouse. Potency of the toxin (as an antigen) induced 6 and 10IU/ml of epsilon and beta anti-toxin in rabbit, respectively. These titers were higher than the minimum level required by the European Pharmacopoeia for epsilon and beta toxins. Experimental challenge with the recombinant fusion toxoid revealed that it could protect mice against C. perfringens epsilon and beta toxins. Toxicity of the fusion toxin was studied by histopathological findings, which were the same as the native toxins. In conclusion, E. coli is a suitable expression host for immunogenic epsilon-beta fusion toxin of C. perfringens.
产气荚膜梭菌是一种厌氧产芽孢、致病的细菌，可导致人类和家畜发生严重疾病。在本研究中，ε-β融合毒素在大肠杆菌中作为可溶性蛋白表达，并用重组细胞裂解物进行了小鼠免疫研究。该毒素（作为抗原）的效价在兔中分别诱导产生了 6 和 10IU/ml 的ε和β抗毒素，高于欧洲药典对ε和β毒素的最低要求。用重组融合类毒素进行的实验性攻毒试验表明，它可以保护小鼠免受产气荚膜梭菌ε和β毒素的侵害。通过组织病理学发现研究了融合毒素的毒性，其与天然毒素相同。总之，大肠杆菌是产气荚膜梭菌免疫原性ε-β融合毒素的合适表达宿主。
